지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 88 - 94 (7page)
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초록· 키워드
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The construction of biosensors with antibodies as the biological sensing element requires the integration of antibodies into the biosensor such that their activity is reproducibly retained. In this work, a peptide ligand was identified from phage-displayed heptameric peptide libraries for the purpose of antibody immobilization on biosensor surfaces. For biopanning of peptides specific to the Fc region of the antibody, rabbit anti-goat immunoglobulin G (IgG) antibody was immobilized on a culture dish coated with goat IgG, which enabled exposure of the Fc region of rabbit antibody on the surface with the Fab region bound to the surface-adsorbed goat IgG. The library phages were added to the dish and phages displaying antibody-binding peptides were competitively eluted using the rabbit antibody. After four rounds of biopanning, 22 phage plaques were randomly selected and their nucleotide sequences corresponding to the random peptide were determined. Three enriched sequences were selected and analyzed for their rabbit antibody-binding activity. One of these sequences (KHRFNKD), which was rich in positive charge and homologous to the IgG-binding domains of Staphylococcal protein A, exhibited a high and specific affinity to rabbit antibody. This peptide was immobilized on a quartz crystal microbalance (QCM) biosensor, and the resulting QCM was found to be capable of capturing antibody and detecting antigen, with potential applications in antibody purification and enzyme immunoassay.
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등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
Lu, H. C. / 2000 / A reusable and specific protein A-coated piezoelectric biosensor for flow injection immunoassay / Biotechnol. Prog 16:116~124
Lee, W. / 2003 / Fabrication of self-assembled protein A monolayer and its application as an immunosensor / Biosens. Bioelectron 19:185~192
Chung, J. W. / 2006 / Immunosensor with a controlled orientation of antibodies by using NeutrAvidin-protein A complex at immunoaffinity layer / J. Biotechnol 126:325~333
Subramanian, A. / 1996 / Effect of antibody orientation on immunosorbent performance / J. Mol. Recognit 9:528~535
Vareiro, M.M. / 2005 / Surface plasmon fluorescence measurements of human chorionic gonadotrophin: role of antibody orientation in obtaining enhanced sensitivity and limit of detection / Anal. Chem 77:2426~2431
Lee, W. / 2003 / Fabrication of self-assembled protein A monolayer and its application as an immunosensor / Biosens. Bioelectron 19:185~192
Chung, J. W. / 2006 / Immunosensor with a controlled orientation of antibodies by using NeutrAvidin-protein A complex at immunoaffinity layer / J. Biotechnol 126:325~333
Subramanian, A. / 1996 / Effect of antibody orientation on immunosorbent performance / J. Mol. Recognit 9:528~535
Vareiro, M.M. / 2005 / Surface plasmon fluorescence measurements of human chorionic gonadotrophin: role of antibody orientation in obtaining enhanced sensitivity and limit of detection / Anal. Chem 77:2426~2431
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