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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 167 - 173 (7page)
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초록· 키워드
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Highly pathogenic avian influenza (HPAI) viruses cause economic losses in the poultry industry and pose a severe threat to human health. Rapid and accurate diagnostic methods are important because they can help prevent further spread of the virus and reduce the time required for eradication of the virus. We developed a low-density microarray for the rapid detection and identification of avian influenza virus subtypes H5, H7, and H9 and their pathotypes in a previous study. In the present study, we report the development of updated probe sets and evaluation of the diagnostic microarray using H5N8 clade 2.3.4.4 HPAI viruses including clinical samples, without the need for egg propagation. Cy3- labeled DNA targets were obtained by reverse transcription polymerase chain reaction using Cy3-labeled universal primers, and labeled amplicons were hybridized to the microarray. All positive samples from RT-PCR showed H5-specific and highly pathogenic pattern in the microarray, without purification of PCR products. Furthermore, it allowed for specific detection of the subtype and pathotype from low DNA concentration samples that did not allow direct sequence analysis. Therefore, this diagnostic microarray has enormous potential for the rapid subtyping and pathotyping from clinical samples without the need for culture.
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참고문헌 (20)
참고문헌 신청
Sonnberg, S. / 2013 / Natural history of highly pathogenic avian influenza H5N1 / Virus Res 178:63~77
Lee, Y.J. / 2014 / Novel reassortant influenza A (H5N8)viruses, South Korea, 2014 / Emerg. Infect. Dis 20:1087~1089
Hanna, A. / 2015 / Genetic Characterization of Highly Pathogenic Avian Influenza (H5N8) Virus from Domestic Ducks, England, November 2014 / Emerg. Infect. Dis 21:879~882
Harder, T. / 2015 / Influenza A(H5N8) Virus Similar to Strain in Korea Causing Highly Pathogenic Avian Influenza in Germany / Emerg. Infect. Dis 21:860~863
Lee, D.H. / 2015 / Intercontinental Spread of Asian-Origin H5N8 to North America through Beringia by Migratory Birds / J. Virol 89:6521~6524
Lee, Y.J. / 2014 / Novel reassortant influenza A (H5N8)viruses, South Korea, 2014 / Emerg. Infect. Dis 20:1087~1089
Hanna, A. / 2015 / Genetic Characterization of Highly Pathogenic Avian Influenza (H5N8) Virus from Domestic Ducks, England, November 2014 / Emerg. Infect. Dis 21:879~882
Harder, T. / 2015 / Influenza A(H5N8) Virus Similar to Strain in Korea Causing Highly Pathogenic Avian Influenza in Germany / Emerg. Infect. Dis 21:860~863
Lee, D.H. / 2015 / Intercontinental Spread of Asian-Origin H5N8 to North America through Beringia by Migratory Birds / J. Virol 89:6521~6524
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