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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 321 - 332 (12page)
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The optimal cell culture method of autologous oral mucosal epithelial cell sheet is not well established for a safe transplantation on to the patients’ ocular surface. Animal serum and 3T3 mouse feeder cells are currently being used to stimulate the growth of the epithelial cells. However, the use of animal compounds can have potential side effects for the patient after transplantation of the engineered cell sheet. In the present study, we focused on engineering a rabbit oral mucosal epithelial cell sheet without 3T3 mouse feeder cells using a mix of Dulbecco’s Modified Eagle Medium/Bronchial Epithelial Cell Growth Medium culture media (DMEM/BEGM). Autologous oral mucosal epithelial cell sheets, engineered with DMEM/BEGM feeder cell free culture media, were compared to those cultured in presence of serum and feeder cells. Using a DMEM/BEGM mix culture media, feeder cell free culture condition, autologous oral mucosal epithelial cells reached confluence and formed a multilayered sheet. The phenotype of engineered cell sheets cultured with DMEM/BEGM were characterized and compared to those cultured with serum and feeder. Hematoxylin and eosin staining showed the formation of a similar stratified multilayer cell sheets, in both culture conditions. The expression of deltaN-p63, ABCG2, PCNA, E-cadherin, Beta-catenin, CK3, CK4, CK13, Muc5AC, was similar in both culture conditions. We demonstrated that rabbit autologous oral mucosal epithelial cell sheet can be engineered, in feeder cell free conditions. The use of the DMEM/BEGM culture media to engineer culture autologous oral mucosa epithelial cell sheet will help to identify key factors involved in the growth and differentiation of oral mucosal epithelial cells.
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참고문헌 (50)
참고문헌 신청
Tseng SC / 1989 / Concept and application of limbal stem cells / Eye (Lond) 3:141~157
Bertalanffy FD / 1962 / Mitotic rate and renewal time of the corneal epithelium in the rat / Arch Ophthalmol 68:546~550
Puangsricharern V / 1995 / Cytologic evidence of corneal diseases with limbal stem cell deficiency / Ophthalmology 102:1476~1485
Haddad A / 2000 / Renewal of the rabbit corneal epithelium as investigated by autoradiography after intravitreal injection of 3Hthymidine / Cornea 19:378~383
Ahmad S / 2012 / Concise review: limbal stem cell deficiency, dysfunction, and distress / Stem Cells Transl Med 1:110~115
Bertalanffy FD / 1962 / Mitotic rate and renewal time of the corneal epithelium in the rat / Arch Ophthalmol 68:546~550
Puangsricharern V / 1995 / Cytologic evidence of corneal diseases with limbal stem cell deficiency / Ophthalmology 102:1476~1485
Haddad A / 2000 / Renewal of the rabbit corneal epithelium as investigated by autoradiography after intravitreal injection of 3Hthymidine / Cornea 19:378~383
Ahmad S / 2012 / Concise review: limbal stem cell deficiency, dysfunction, and distress / Stem Cells Transl Med 1:110~115
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