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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한백신학회 Clinical and Experimental Vaccine Research Clinical and Experimental Vaccine Research Vol.7 No.1
- 발행연도
- 2018.1
- 수록면
- 61 - 69 (9page)
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초록· 키워드
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Purpose: The first aim of this study was to develop a novel inactivated porcine epidemic diarrhea virus (PEDV) vaccine using the recently isolated Korean PEDV QIAP1401 strain and to evaluate its protective efficacy in growing pigs. The second was to determine the optimum adjuvant formulation of the inactivated PEDV vaccine that induces protection against viral challenge. Materials and Methods: To generate high titers of infectious PEDV, the QIAP1401 isolate was passaged in Vero cells. The experimental vaccines were prepared from a binary ethyleneimine-inactivated QIAP1401 strain passaged sequentially 70 times (QIAP1401-p70), formulated with four commercial adjuvants, and administered twice intramuscularly to growing pigs. Challenge studies using a virulent homologous strain of PEDV QIAP1401-p11, which was passaged 11 times after isolation, were performed to assess protection against disease progression and viral shedding during the 15-day observation period. The vaccine-induced antibody responses were measured in serum samples collected at predetermined time points by indirect enzyme-linked immunosorbent assay and virus neutralization test. Results: The QIAP1401-p70 strain had 42 amino acid (aa) mutations, including a 25 aa deletion, and was selected as the inactivated PEDV vaccine candidate. Although none of the pigs that received the experimental vaccines were completely protected against subsequent viral challenge, they exhibited a significantly higher immune response than did non-vaccinated control pigs. Among the vaccine groups, the highest antibody responses were observed in the pigs that received an oil-based multiphasic water/oil/water (W/O/W) emulsion adjuvanted vaccine, which delayed the onset of clinical symptoms and viral shedding. Conclusion: A novel inactivated PEDV vaccine formulated with a W/O/W emulsion adjuvant was both immunogenic and protective against viral challenge.
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참고문헌 (35)
참고문헌 신청
Debouck P / 1980 / Experimental infection of pigs with a new porcine enteric coronavirus, CV 777 / Am J Vet Res 41:219~223
Pensaert MB / 2006 / Disease of swine / Blackwell Publishing :367~372
Pijpers A / 1993 / Porcine epidemic diarrhoea virus as a cause of persistent diarrhoea in a herd of breeding and finishing pigs / Vet Rec 132:129~131
Oldham J / 1972 / Letter to the editor / Pig Farming 10:72~73
Pensaert MB / 1978 / A new coronavirus-like particle associated with diarrhea in swine / Arch Virol 58:243~247
Pensaert MB / 2006 / Disease of swine / Blackwell Publishing :367~372
Pijpers A / 1993 / Porcine epidemic diarrhoea virus as a cause of persistent diarrhoea in a herd of breeding and finishing pigs / Vet Rec 132:129~131
Oldham J / 1972 / Letter to the editor / Pig Farming 10:72~73
Pensaert MB / 1978 / A new coronavirus-like particle associated with diarrhea in swine / Arch Virol 58:243~247
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