지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 418 - 424 (7page)
- DOI
- 10.7783/KJMCS.2021.29.6.418
이용수
초록· 키워드
오류제보하기
Background: Although Astragalus membranaceus is a major medicinal herb used for food and medicinal purposes, genome-based research for its exploration is insufficient. The introduction of the genetic breeding system for medicinal crops, based on genomic analysis, will have a great impact not only on the establishment of the origin but also on understanding the existing traditional breeding system.
Methods and Results: A. membranaceus from five accessions of Poongsung, Asung, Jecheon, Sancheong, and Jilin from China were used, which are cultivated in the test field. The produced nucleotide sequences were assembled de novo to obtain 450,449 contigs, and the repeat sequences were extracted using the selection criteria, two to six nucleotide sequences repeated five time or more. Finally, we obtained 147,766 simple sequence repeat (SSR) marker candidates. Using the CLC genomics workbench program, 949 SSR markers showing mutations for Astragalus in the 5 accessions were selected. From the designed primer sets, 99 were randomly selected and applied to the genomic DNA which was extracted from five cultivars and pooled. Ten primer sets showing more than two bands were finally selected as SSR markers, and were used for the genetic relationship analysis in 81 strains of A. membranaceus.
Conclusions: The data of this study can be used as a marker when registering A. membranaceus varieties through pure line selection and line breeding in the future. It is thought that it can greatly contribute to securing intellectual property rights for domestic Astragalus breeding varieties.
Methods and Results: A. membranaceus from five accessions of Poongsung, Asung, Jecheon, Sancheong, and Jilin from China were used, which are cultivated in the test field. The produced nucleotide sequences were assembled de novo to obtain 450,449 contigs, and the repeat sequences were extracted using the selection criteria, two to six nucleotide sequences repeated five time or more. Finally, we obtained 147,766 simple sequence repeat (SSR) marker candidates. Using the CLC genomics workbench program, 949 SSR markers showing mutations for Astragalus in the 5 accessions were selected. From the designed primer sets, 99 were randomly selected and applied to the genomic DNA which was extracted from five cultivars and pooled. Ten primer sets showing more than two bands were finally selected as SSR markers, and were used for the genetic relationship analysis in 81 strains of A. membranaceus.
Conclusions: The data of this study can be used as a marker when registering A. membranaceus varieties through pure line selection and line breeding in the future. It is thought that it can greatly contribute to securing intellectual property rights for domestic Astragalus breeding varieties.
목차
ABSTRACT
서언
재료 및 방법
결과 및 고찰
REFERENCES
참고문헌 (31)
참고문헌 신청
Dong TTX / 2003 / Phylogeny of Astragalus in China : Molecular evidence from the DNA sequences of 5S rRNA spacer, ITS, and 18S rRNA / Journal of Agricultural and Food Chemistry 51:6709~6714
Du X / 2012 / Astragalus polysaccharides enhance immune responses of HBV DNA vaccination via promoting the dendritic cell maturation and suppressing Treg frequency in mice / International Immunopharmacology 14:463~470
Fu J / 2014 / Review of the botanical characteristics, phytochemistry, and pharmacology of Astragalus membranaceus(Huangqi) / Phytotherapy Research 28:1275~1283
Gil JS / 2017 / Development of genome-wide SSR markers from Angelica gigas Nakai using next generation sequencing / Genes 8:238
Henry RJ / 2012 / Next-generation sequencing for understanding and accelerating crop domestication / Briefings in Functional Genomics 11:51~56
Du X / 2012 / Astragalus polysaccharides enhance immune responses of HBV DNA vaccination via promoting the dendritic cell maturation and suppressing Treg frequency in mice / International Immunopharmacology 14:463~470
Fu J / 2014 / Review of the botanical characteristics, phytochemistry, and pharmacology of Astragalus membranaceus(Huangqi) / Phytotherapy Research 28:1275~1283
Gil JS / 2017 / Development of genome-wide SSR markers from Angelica gigas Nakai using next generation sequencing / Genes 8:238
Henry RJ / 2012 / Next-generation sequencing for understanding and accelerating crop domestication / Briefings in Functional Genomics 11:51~56
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