지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 197 - 204 (8page)
이용수
초록· 키워드
오류제보하기
Purpose: Electroporation is known to enhance the efficiency of gene transfer through a transient increase in cell membrane permeability. The aim of this study was to determine the optimal conditions for in vivo electroporation-mediated gene delivery into mouse corpus cavernosum.
Materials and Methods: Diabetes was induced in C57BL/6 mice by intraperitoneal injections of streptozotocin. After intracavernous injection of pCMV-Luc (100 μg/40 μL), different electroporation settings (5–50 V, 8–16 pulses with a duration of 40–100 ms) were applied to the penis to establish the optimal conditions for electroporation. Gene expression was evaluated by luciferase assay. We also assessed the undesired consequences of electroporation by visual inspection and hematoxylin-eosin staining of penile tissue.
Results: Electroporation profoundly induced gene expression in the corpus cavernosum tissue of normal mice in a voltage-dependent manner. We observed electrical burn scars in the penis of normal mice who received electroporation with eight 40-ms pulses at a voltage of 50 V and sixteen 40-ms pulses, eight 100-ms pulses, and sixteen 100-ms pulses at a voltage of 30 V. No detectable burn scars were noted in normal mice stimulated with eight 40-ms pulses at a voltage of 30 V. Electroporation also significantly induced gene expression in diabetic mice stimulated with 40-ms pulse at a voltage of 30 V without injury to the penis.
Conclusions: We have established the optimal electroporation conditions for maximizing gene transfer into the corpus cavernosum of mice while avoiding damage to the erectile tissue. The electroporation-mediated gene delivery technique will be a valuable tool for gene therapy in the field of erectile dysfunction.
목차
등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Goldstein I / 1998 / Oral sildenafil in the treatment of erectile dysfunction. Sildenafil Study Group / N Engl J Med 338:1397~1404
Rendell MS / 1999 / Sildenafil for treatment of erectile dysfunction in men with diabetes: a randomized controlled trial. Sildenafil Diabetes Study Group / JAMA 281:421~426
최형기 / 2006 / 한국의 발기부전 환자에 필요 시 투여된 타다나필의 효능과 안전성에 대한 이중 맹검시험 / Investigative and Clinical Urology 47(8):852~858
Martinez-Jabaloyas JM / 2001 / Prognostic factors for response to sildenafil in patients with erectile dysfunction / Eur Urol 40:641~646
Gresser U / 2002 / Erectile dysfunction: comparison of efficacy and side effects of the PDE-5 inhibitors sildenafil, vardenafil and tadalafil: review of the literature / Eur J Med Res 7:435~446
Rendell MS / 1999 / Sildenafil for treatment of erectile dysfunction in men with diabetes: a randomized controlled trial. Sildenafil Diabetes Study Group / JAMA 281:421~426
최형기 / 2006 / 한국의 발기부전 환자에 필요 시 투여된 타다나필의 효능과 안전성에 대한 이중 맹검시험 / Investigative and Clinical Urology 47(8):852~858
Martinez-Jabaloyas JM / 2001 / Prognostic factors for response to sildenafil in patients with erectile dysfunction / Eur Urol 40:641~646
Gresser U / 2002 / Erectile dysfunction: comparison of efficacy and side effects of the PDE-5 inhibitors sildenafil, vardenafil and tadalafil: review of the literature / Eur J Med Res 7:435~446
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