지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 154 - 161 (8page)
이용수
초록· 키워드
오류제보하기
Background Natural killer (NK) cells constantly survey surrounding tissues and remove newly generated cancer cells, independent of cancer antigen recognition. Although there have been a number of attempts to apply NK cells for cancer therapy, clinical application has been somewhat limited because of the difficulty in preparing a sufficient number of NK cells.
Therefore, ex vivo NK cell expansion is one of the important steps for developing NK cell therapeutics.
Methods CD3+ depleted lymphocytes were cocultured with IL-2 and with feeder cells (peripheral blood mononuclear cells [PBMCs], K562, and Jurkat) for 15 days. Expanded NK cells were tested for cytotoxicity against cancer cell lines.
Results We compared feeder activities of three different cells−PBMC, K562, and Jurkat. K562 expanded NK cells by almost 20 fold and also showed powerful cytotoxic activity against cancer cells. K562-NK cells remarkably expressed the NK cell activation receptors, NKG2D, and DNAM-1. K562-NK cells exhibited more than two-fold production of cytotoxic granules compared with Jurkat-NK cells, producing more perforin and granzyme B than naïve NK cells.
Conclusion Our findings suggest that K562 are more efficient feeder cells than Jurkat or PBMCs. K562 feeder cells expanded NK cells by almost 20 fold and showed powerful cytotoxic activity against cancer cells. We herein propose an intriguing approach for a design of NK cell expansion.
목차
등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Vivier E / 2008 / Functions of natural killer cells / Nat Immunol 9:503~510
Perussia B / 1987 / Preferential proliferation of natural killer cells among peripheral blood mononuclear cells cocultured with B lymphoblastoid cell lines / Nat Immun Cell Growth Regul 6:171~188
Moretta A / 2002 / Natural killer cells and dendritic cells: rendezvous in abused tissues / Nat Rev Immunol 2:957~964
Moretta A / 2001 / Activating receptors and coreceptors involved in human natural killer cell-mediated cytolysis / Annu Rev Immunol 19:197~223
Houchins JP / 1991 / DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells / J Exp Med 173:1017~1020
Perussia B / 1987 / Preferential proliferation of natural killer cells among peripheral blood mononuclear cells cocultured with B lymphoblastoid cell lines / Nat Immun Cell Growth Regul 6:171~188
Moretta A / 2002 / Natural killer cells and dendritic cells: rendezvous in abused tissues / Nat Rev Immunol 2:957~964
Moretta A / 2001 / Activating receptors and coreceptors involved in human natural killer cell-mediated cytolysis / Annu Rev Immunol 19:197~223
Houchins JP / 1991 / DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells / J Exp Med 173:1017~1020
이 논문의 저자 정보
최근 본 자료
댓글(0)
0