지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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We separated an Aw10 allele by allele-specific sequencing in two Aw10/B101 samples that had the AweakB phenotype. Two samples with the A102/B101 genotype were also tested as a control. The reverse primers using position 930 at exon7 were designed for allele-specific sequencing. The differential positions were a total of 52 points for distinguishing the A-allele from the B-allele. Although overlaps with another haplotype allele that showed a minor chromatographic peak were observed in almost all the points, the specific allele-separation rate was 100% (52/52) by assessing the dominance in the chromatographic peak height. Based on the separation rate in the two cases with Aw10/B101 and the two AB controls, allele-specific sequencing is a convenient and reliable method for the separating the A-allele and B-allele in a clinical laboratory.
목차
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참고문헌 (15)
참고문헌 신청
Yamamoto F / 1990 / Molecular genetic basis of the histo-blood group ABO system / Nature 345:229~233
Seltsam A / 2003 / Systematic analysis of the ABO gene diversity within exons 6 and 7 by PCR screening reveals new ABO alleles / Transfusion 43:428~439
Olsson ML / 2001 / Genomic analysis of clinical samples with serologic ABO blood grouping discrepancies: identification of 15 novel A and B subgroup alleles / Blood 98:1585~1593
Ogasawara K / 1996 / Molecular genetic analysis of variant phenotypes of the ABO blood group system / Blood 88:2732~2737
Cho D / 2004 / The serological and genetic basis of the cis-AB blood group in Korea / Vox Sang 87:41~43
Seltsam A / 2003 / Systematic analysis of the ABO gene diversity within exons 6 and 7 by PCR screening reveals new ABO alleles / Transfusion 43:428~439
Olsson ML / 2001 / Genomic analysis of clinical samples with serologic ABO blood grouping discrepancies: identification of 15 novel A and B subgroup alleles / Blood 98:1585~1593
Ogasawara K / 1996 / Molecular genetic analysis of variant phenotypes of the ABO blood group system / Blood 88:2732~2737
Cho D / 2004 / The serological and genetic basis of the cis-AB blood group in Korea / Vox Sang 87:41~43
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