지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2023.2
- 수록면
- 12 - 17 (6page)
- DOI
- 10.7783/KJMCS.2023.31.1.12
이용수
초록· 키워드
오류제보하기
Background: Three medicinal plants of the Campanulaceae, Codonopsis lanceolata, C. pilosula, and C. ussuriensis are perennial vines of the genus Codonopsis, and are widely disturbuted in East Asia. C. lanceolata, C. pilosula, and C. ussuriensis have been used as medicine to alleviate inflammation, increase red blood cell count and hemoglobin levels, and improve spleen and stomach functions, respectively. The three species have similar morphologies, although their pharmacological effects differ.
Methods and Results: We identified polymorphic loci by comparing chloroplast and mitochondrial sequences of C. lanceolata, C. pilosula, and C. ussuriensis. We designed primers based on these polymorphic loci and conducted polymerase chain reaction (PCR) analysis to verify the polymorphisms. Three insertion–deletion (InDel) markers were ultimately developed: CD-mt-02, which identified C. pilosula; CD-mt-06, which identified C. ussuriensis; and CD-cp-07, which identified all the three species.
Conclusions: The three InDel markers developed in this study easily distinguished the three plant species based on a simple PCR test.
Methods and Results: We identified polymorphic loci by comparing chloroplast and mitochondrial sequences of C. lanceolata, C. pilosula, and C. ussuriensis. We designed primers based on these polymorphic loci and conducted polymerase chain reaction (PCR) analysis to verify the polymorphisms. Three insertion–deletion (InDel) markers were ultimately developed: CD-mt-02, which identified C. pilosula; CD-mt-06, which identified C. ussuriensis; and CD-cp-07, which identified all the three species.
Conclusions: The three InDel markers developed in this study easily distinguished the three plant species based on a simple PCR test.
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ABSTRACT
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재료 및 방법
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REFERENCES
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UCI(KEPA) : I410-ECN-0101-2023-524-000441539