DsLCYB Directionally Modulated β-Carotene of the Green Alga Dunaliella salina Under Red Light Stress

Lan Yanhong; Song Yao; Guo Yihan; Qiao Dairong; Cao Yi; Xu Hui

doi:10.4014/jmb.2208.08044

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자료유형: 학술저널

저자정보: Lan Yanhong (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province College of Life Sciences) Song Yao (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province College of Life Sciences) Guo Yihan (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province College of Life Sciences) Qiao Dairong (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province College of Life Sciences) Cao Yi (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province College of Life Sciences) Xu Hui (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province College of Life Sciences)

저널정보: 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제12호

발행연도: 2022.12

수록면: 1,622 - 1,631 (10page)

DOI: 10.4014/jmb.2208.08044

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Carotenoids, which are natural pigments found abundantly in wide-ranging species, have diverse functions and high industrial potential. The carotenoid biosynthesis pathway is very complex and has multiple branches, while the accumulation of certain metabolites often affects other metabolites in this pathway. The DsLCYB gene that encodes lycopene cyclase was selected in this study to evaluate β-carotene production and the accumulation of β-carotene in the alga Dunaliella salina. Compared with the wild type, the transgenic algal species overexpressed the DsLCYB gene, resulting in a significant enhancement of the total carotenoid content, with the total amount reaching 8.46 mg/g for an increase of up to 1.26-fold. Interestingly, the production of α-carotene in the transformant was not significantly reduced. This result indicated that the regulation of DsLCYB on the metabolic flux distribution of carotenoid biosynthesis is directional. Moreover, the effects of different light-quality conditions on β-carotene production in D. salina strains were investigated. The results showed that the carotenoid components of β-carotene and β-cryptoxanthin were 1.8- fold and 1.23-fold higher than that in the wild type under red light stress, respectively. This suggests that the accumulation of β-carotene under red light conditions is potentially more profitable.

#β-Carotene #lycopene β cyclase #carotenoid #Dunaliella salina #transgene

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