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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.1
- 수록면
- 47 - 53 (7page)
- DOI
- 10.3343/alm.2022.42.1.47
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초록· 키워드
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Background: Since 2017, automated assays have been used in most clinical laboratories for anti-Mullerian hormone (AMH) level measurement. We evaluated the analytical performance of the newly developed automated fluorescent immunoassay system (AFIAS) AMH assay (Boditech Med, Gangwon-do, Korea) in comparison with the Roche Elecsys and Beckman Coulter Access 2 AMH assays.
Methods: Analytical performance of the AFIAS AMH assay was assessed in terms of linearity, repeatability, and within-laboratory precision (CV%) using human recombinant AMH samples according to the Clinical and Laboratory Standards Institute (CLSI) guidelines EP05 and EP06. Using 293 serum samples collected from an infertility clinic, the AMH levels were compared across AFIAS, Elecsys, and Access 2 AMH assays according to the CLSI EP09 guidelines.
Results: The AFIAS AMH assay results were linear across the measurement range of 0.420?72.386 pmol/L AMH, with repeatability of 6.341%. CV% of the AFIAS AMH assay for three levels of control, 1.786, 7.143, and 56.857 pmol/L, were 5.801%, 5.714%, and 6.228%, respectively. The results of the three AMH assays showed strong correlation: AFIAS and Elecsys [slope, 1.055 (95% confidence interval (CI), 1.022?1.088) and Spearman’s rho, 0.978 (95% CI, 0.973?0.983)], Elecsys and Access 2 [slope, 0.813 (95% CI, 0.791?0.834) and Spearman’s rho, 0.986 (95% CI, 0.983?0.989)], and AFIAS and Access 2 [slope, 0.836 (95% CI, 0.821?0.853) and Spearman’s rho, 0.984 (95% CI, 0.980? 0.988)].
Conclusions: The AFIAS AMH assay may be an alternative to the Roche Elecsys and Beckman Coulter Access 2 AMH assays.
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