Heparin Immobilization of Tissue Engineered Xenogeneic Small Diameter Arterial Scaffold Improve Endothelialization

Tardalkar Kishor; Marsale Tejesh; Bhamare Nilesh; Kshersagar Jeevitaa; Chaudhari Leena; Joshi Meghnad G.

doi:10.1007/s13770-021-00411-7

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자료유형: 학술저널

저자정보: Tardalkar Kishor (Department of Stem Cells & Regenerative Medicine D Y Patil Education Society (Deemed University) Marsale Tejesh (Department of Stem Cells & Regenerative Medicine D Y Patil Education Society (Deemed University) Bhamare Nilesh (Department of Stem Cells & Regenerative Medicine D Y Patil Education Society (Deemed University) Kshersagar Jeevitaa (Department of Stem Cells & Regenerative Medicine D Y Patil Education Society (Deemed University) Chaudhari Leena (Department of Stem Cells & Regenerative Medicine D Y Patil Education Society (Deemed University) Joshi Meghnad G. (Department of Stem Cells & Regenerative Medicine D Y Patil Education Society (Deemed University)

저널정보: 한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제19권 제3호

발행연도: 2022.6

수록면: 505 - 523 (19page)

DOI: 10.1007/s13770-021-00411-7

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Background: Autologous vessels graft (Inner diameter?<?6 mm) harvesting always challenged during bypass grafting surgery and its complication shows poor outcome. Tissue engineered vascular graft allow to generate biological graft without any immunogenic complication. The approach presented in this study is to induce graft remodeling through heparin coating in luminal surface of small diameter (Inner diameter?<?1 mm) decellularized arterial graft. Methods: Decellularization of graft was done using SDS, combination of 0.5% sodium dodecyl sulfate and 0.5% sodium deoxycholate and only sodium deoxycholate. Decellularization was confirmed on basis of histology, and DAPI. Characterization of extracellular matrix was analyzed using histology and scanning electron microscopy. Surface modification of decellularized vascular graft was done with heparin coating. Heparin immobilization was evaluated by toluidine blue stain. Heparin-coated graft was transplanted end to end anastomosis in femoral artery in rat. Results: Combination of 0.5% sodium dodecyl sulfate and 0.5% Sodium deoxycholate showed complete removal of xenogeneic cells. The heparin coating on luminal surface showed anti-thrombogenicity and endothelialization. Mechanical testing revealed no significant differences in strain characteristics and modulus between native tissues, decellularized scaffolds and transplanted scaffold. Collectively, this study proposed a heparin-immobilized ECM coating to surface modification offering functionalize biomaterials for developing small-diameter vascular grafts. Conclusion: We conclude that xenogeneic decellularized arterial scaffold with heparin surface modification can be fabricated and successfully transplanted small diameter (inner diameter?<?1 mm) decellularized arterial graft.

#Xenogeneic Vascular graft Decellularization Small diameter vessels Heparin surface modification

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