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논문 기본 정보

자료유형
학술저널
저자정보
Kim Dae-Hee (Department of Biomedical Engineering College of Medicine Seoul National University) Kim Da-Seul (Department of Biomedical Science CHA University) Ha Hyun Jeong (Department of Plastic and Reconstructive Surgery CHA Bundang Medical Center CHA University School) Jung Ji-Won (Department of Plastic and Reconstructive Surgery CHA Bundang Medical Center CHA University School) 백승운 (차의과학대학교) Baek Seung Hwa (CHA Advanced Research Institute Center for Research & Development Histological Analysis Team) 김태형 (중앙대학교) 이정찬 (서울대학교) Hwang Euna (Department of Plastic and Reconstructive Surgery CHA Bundang Medical Center CHA University School) Han Dong Keun (CHA University)
저널정보
한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제19권 제3호
발행연도
2022.6
수록면
553 - 563 (11page)
DOI
10.1007/s13770-021-00426-0

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BACKGROUND: Autologous fat grafting is one of the most common procedures used in plastic surgery to correct soft tissue deficiency or depression deformity. However, its clinical outcomes are often suboptimal, and lack of metabolic and architectural support at recipient sites affect fat survival leading to complications such as cyst formation, calcification. Extracellular matrix-based scaffolds, such as allograft adipose matrix (AAM) and poly(lactic-co-glycolic) acid (PLGA), have shown exceptional clinical promise as regenerative scaffolds. Magnesium hydroxide (MH), an alkaline ceramic, has attracted attention as a potential additive to improve biocompatibility. We attempted to combine fat graft with regenerative scaffolds and analyzed the changes and viability of injected fat graft in relation to the effects of injectable natural, and synthetic (PLGA/MH microsphere) biomaterials. METHODS: In vitro cell cytotoxicity, angiogenesis of the scaffolds, and wound healing were evaluated using human dermal fibroblast cells. Subcutaneous soft-tissue integration of harvested fat tissue was investigated in vivo in nude mouse with random fat transfer protocol Fat integrity and angiogenesis were identified by qRT-PCR and immunohistochemistry. RESULTS: In vitro cell cytotoxicity was not observed both in AAM and PLGA/MH with human dermal fibroblast. PLGA/MH and AAM showed excellent wound healing effect. In vivo, the AAM and PLGA/MH retained volume compared to that in the only fat group. And the PLGA/MH showed the highest angiogenesis and anti-inflammation. CONCLUSION: In this study, a comparison of the volume retention effect and angiogenic ability between autologous fat grafting, injectable natural, and synthetic biomaterials will provide a reasonable basis for fat grafting.

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