개인구독
소속 기관이 없으신 경우, 개인 정기구독을 하시면 저렴하게
논문을 무제한 열람 이용할 수 있어요.
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.3
- 수록면
- 202 - 210 (9page)
이용수
초록· 키워드
오류제보하기
The nonstructural protein 5A (NS5A) encoded by the human hepatitis C virus (HCV) RNA genome is a multifunctional phosphoprotein. To analyse the influence of NS5A on apoptosis, we established an Hep-NS5A cell line (HepG2 cells that stably express NS5A) and induced apoptosis using tumour necrosis factor (TNF)-?. We utilised the MTT assay to detect cell viability, real-time quantitative polymerase chain reaction and Western blot to analyse gene and protein expres-sion, and a luciferase reporter gene experiment to investigate the targeted regulatory relationship. Chromatin immunoprecipitation was used to identify the combination of NF-?B and miR-503. We found that overexpression of NS5A inhibited TNF-?-induced hepatocellular apoptosis via regulating miR-503 expression. The cell viability of the TNF-? induced Hep-mock cells was significantly less than the viability of the TNF-? induced Hep-NS5A cells, which demonstrates that NS5A inhibited TNF-?-induced HepG2 cell apoptosis. Under TNF-? treatment, miR-503 expression was decreased and cell viability and B-cell lymphoma 2 (bcl-2) expression were increased in the Hep-NS5A cells. Moreover, the luciferase reporter gene experiment verified that bcl-2 was a direct target of miR-503, NS5A inhibited TNF-?-induced NF-?B activation and NF-?B regulated miR-503 transcription by combining with the miR-503 promoter. After the Hep-NS5A cells were transfected with miR-503 mimics, the data indicated that the mimics could reverse TNF-?-induced cell apoptosis and blc-2 expression. Collectively, our findings suggest a possible molecular mechanism that may contribute to HCV treatment in which NS5A inhibits NF-?B activation to decrease miR-503 expression and increase bcl-2 expression, which leads to a decrease in hepatocellular apoptosis.
목차
등록된 정보가 없습니다.
참고문헌 (32)
참고문헌 신청
Bandiera, S. / 2016 / Hepatitis C virus-induced upregulation of microRNA miR-146a-5p in hepatocytes promotes viral infection and deregulates metabolic pathways associated with liver disease pathogenesis / J. Virol 90:6387~6400
Bartel, D. P. / 2004 / MicroRNAs: genomics, biogenesis, mechanism, and function / Cell 116:281~297
Caporali, A. / 2015 / P75NTR-dependent activation of NF- B regulates microRNA-503transcription and pericyte-endothelial crosstalk in diabetes after limb ischaemia / Nat. Commun 6:8024
Charlton, M. / 2015 / Ledipasvir and Sofosbuvir Plus Ribavirin for Treatment of HCV Infection in Patients With Advanced Liver Disease / Gastroenterology 149:649~659
Chen, X. / 2012 / CpG island methylation status of miRNAs in esophageal squamous cell carcinoma / Int. J. Cancer 130:1607~1613
Bartel, D. P. / 2004 / MicroRNAs: genomics, biogenesis, mechanism, and function / Cell 116:281~297
Caporali, A. / 2015 / P75NTR-dependent activation of NF- B regulates microRNA-503transcription and pericyte-endothelial crosstalk in diabetes after limb ischaemia / Nat. Commun 6:8024
Charlton, M. / 2015 / Ledipasvir and Sofosbuvir Plus Ribavirin for Treatment of HCV Infection in Patients With Advanced Liver Disease / Gastroenterology 149:649~659
Chen, X. / 2012 / CpG island methylation status of miRNAs in esophageal squamous cell carcinoma / Int. J. Cancer 130:1607~1613
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
MiR-1299 functions as a tumor suppressor to inhibit the proliferation and metastasis of gastric cancer by targeting ARF6
Genes & Genomics
2022 .02
-
Bioinformatic identification and expression analysis of the chicken B cell lymphoma (BCL) gene
Genes & Genomics
2019 .01
-
The Effect of miR-361-3p Targeting TRAF6 on Apoptosis of Multiple Myeloma Cells
Journal of Microbiology and Biotechnology
2021 .01
-
Protective Role of miR-34c in Hypoxia by Activating Autophagy through BCL2 Repression
Molecules and Cells
2022 .06
-
The seed sequence is necessary but insufficient for downregulation of target genes by miR-608
Genes & Genomics
2016 .01
-
Circulating microRNA expression profiling in young obese Korean women
Nutrition Research and Practice
2020 .08
-
Comparison of the miR-23b and miR-203 Expressions in Endometrial Cancer
대한임상검사과학회지
2017 .01
-
Overexpression of Long Non-Coding RNA MIR22HG Represses Proliferation and Enhances Apoptosis via miR-629-5p/TET3 Axis in Osteosarcoma Cells
Journal of Microbiology and Biotechnology
2021 .10
-
miR-30a Regulates the Expression of CAGE and p53 and Regulates the Response to Anti-Cancer Drugs
Molecules and Cells
2016 .04
-
MiR-339 attenuates LPS-induced intestinal epithelial cells inflammatory responses and apoptosis by targeting TLR4
Genes & Genomics
2020 .01
-
MicroRNA-183 accelerates the proliferation of hepatocyte during liver regeneration through targeting programmed cell death protein 6
Genes & Genomics
2022 .08
-
LncRNA H19 Drives Proliferation of Cardiac Fibroblasts and Collagen Production via Suppression of the miR-29a-3p/miR-29b-3p-VEGFA/TGF-β Axis
Molecules and Cells
2022 .03
-
IKKγ Facilitates the Activation of NF-κB by Hepatitis C Virus Core Protein
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2018 .09
-
MiR-200c-3p inhibits LPS-induced M1 polarization of BV2 cells by targeting RIP2
Genes & Genomics
2022 .04
-
Suppression of HELLS by miR-451a represses mTOR pathway to hinder aggressiveness of SCLC
Genes & Genomics
2021 .01
-
Circulating microRNA expression profiling in young obese Korean women
Nutrition Research and Practice
2020 .08
-
The Applications of Hepatitis C Virus (HCV) Replication System in Developing Anti-HCV Reagents
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2015 .06
-
Hepatitis C Virus Nonstructural 5A Protein Interacts with Telomere Length Regulation Protein: Implications for Telomere Shortening in Patients Infected with HCV
Molecules and Cells
2022 .03
-
miRNA-103a-3p Promotes Human Gastric Cancer Cell Proliferation by Targeting and Suppressing ATF7 in vitro
Molecules and Cells
2018 .03
-
LINC01272 Suppressed Cell Multiplication and Induced Apoptosis via Regulating MiR-7-5p/CRLS1 Axis in Lung Cancer
Journal of Microbiology and Biotechnology
2021 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0