Overexpression of microRNA-612 Restrains the Growth, Invasion, and Tumorigenesis of Melanoma Cells by Targeting Espin

Ying Zhu; Hao-liang Zhang; Qi-ying Wang; Min-jing Chen; Lin-bo Liu

doi:10.14348/molcells.2018.2235

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자료유형: 학술저널

저자정보: Ying Zhu (The First Affiliated Hospital of Zhengzhou University) Hao-liang Zhang (The First Affiliated Hospital of Zhengzhou University) Qi-ying Wang (The First Affiliated Hospital of Zhengzhou University) Min-jing Chen (The First Affiliated Hospital of Zhengzhou University) Lin-bo Liu (The First Affiliated Hospital of Zhengzhou University)

저널정보: 한국분자세포생물학회 Molecules and Cells Molecules and Cells 제41권 제2호

발행연도: 2018.2

수록면: 119 - 126 (8page)

DOI: 10.14348/molcells.2018.2235

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microRNA (miR)-612 shows anticancer activity in several types of cancers, yet its function in melanoma is still unclear. This study was undertaken to investigate the expression of miR-612 and its biological relevance in melanoma cell growth, invasion, and tumorigenesis. The expression and prognostic significance of miR-612 in melanoma were examined. The effects of miR-612 overexpression on cell proliferation, colony formation, tumorigenesis, and invasion were determined. Rescue experiments were conducted to identify the functional target gene(s) of miR-612. miR-612 was significantly downregulated in melanoma tissues compared to adjacent normal tissues. Low miR-612 expression was significantly associated with melanoma thickness, lymph node metastasis, and shorter overall, and disease-free survival of patients. Overexpression of miR-612 significantly decreased cell proliferation, colony formation, and invasion of SK-MEL-28 and A375 melanoma cells. In vivo tumorigenic studies confirmed that miR-612 overexpression retarded the growth of A375 xenograft tumors, which was coupled with a decline in the percentage of Ki-67-positive proliferating cells. Mechanistically, miR-612 targeted Espin in melanoma cells. Overexpression of Espin counteracted the suppressive effects of miR-612 on melanoma cell proliferation, invasion, and tumorigenesis. A significant inverse correlation (r = -0.376, P = 0.018) was observed between miR-612 and Espin protein expression in melanoma tissues. In addition, overexpression of miR-612 and knockdown of Espin significantly increased the sensitivity of melanoma cells to doxorubicin. Collectively, miR-612 suppresses the aggressive phenotype of melanoma cells through downregulation of Espin. Delivery of miR-612 may represent a novel therapeutic strategy against melanoma.

#aggressiveness #downregulation #metastasis #microRNA #target

참고문헌 (25)

참고문헌 신청

Alaoui-Jamali, M.A. / 2009 / A novel experimental heme oxygenase-1-targeted therapy for hormonerefractory prostate cancer / Cancer Res. 69:8017~8024

Deacu, E. / 2004 / Activin type II receptor restoration in ACVR2-deficient colon cancer cells induces transforming growth factor-beta response pathway genes / Cancer Res. 64:7690~7696

Donaudy, F. / 2006 / Espin gene (ESPN) mutations associated with autosomal dominant hearing loss cause defects in microvillar elongation or organisation / J. Med. Genet. 43:157~161

Cheerla, N. / 2017 / MicroRNA based Pan-Cancer diagnosis and treatment recommendation / BMC Bioinformatics 18:32

Fahrioglu, U. / 2016 / Ferulic acid decreases cell viability and colony formation while inhibiting migration of MIA PaCa-2 human pancreatic cancer cells in vitro / Gene 576:476~482

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