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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 510 - 518 (9page)
- DOI
- 10.48022/mbl.2109.09013
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초록· 키워드
오류제보하기
Bacteriophages are considered excellent sensing elements for platforms detecting bacteria. However, their lytic cycle has restricted their efficacy. Here, we used the minor coat protein pIII domain (N1N2) of phage CTXφ to construct a novel surface plasmon resonance (SPR) biosensor that could detect Vibrio cholerae.
N1N2 harboring the domains required for phage adsorption and entry was obtained from Escherichia coli using recombinant protein expression and purification. SDS-PAGE revealed an approximate size of 30 kDa for N1N2. Dot blot and transmission electron microscopy analyses revealed that the protein bound to the host V. cholerae but not to non-host E. coli K-12 cells. Next, we used amine-coupling to develop a novel recombinant N1N2 (rN1N2)?functionalized SPR biosensor by immobilizing rN1N2 proteins on gold substrates and using SPR to monitor the binding kinetics of the proteins with target bacteria. We observed rapid detection of V. cholerae in the range of approximately 103 to 109 CFU/ml but not of E. coli at any tested concentration, thereby confirming that the biosensor exhibited differential recognition and binding. The results indicate that the novel biosensor can rapidly monitor a target pathogenic microorganism in the environment and is very useful for monitoring food safety and facilitating early disease prevention.
목차
등록된 정보가 없습니다.
참고문헌 (33)
참고문헌 신청
Bonnin-Jusserand M / 2019 / Vibrio species involved in seafood-borne outbreaks (Vibrio cholerae, V. parahaemolyticus and V. vulnificus): Review of microbiological versus recent molecular detection methods in seafood products / Crit. Rev. Food Sci. Nutr 59:597~610
Faruque SM / 2012 / Phage-bacterial interactions in the evolution of toxigenic Vibrio cholerae / Virulence 3:556~565
Lazcka O / 2007 / Pathogen detection: a perspective of traditional methods and biosensors / Biosens. Bioelectron 22:1205~1217
Dwivedi HP / 2011 / Detection of pathogens in foods:the current state-of-the-art and future directions / Crit. Rev. Microbiol 37:40~63
D'Souza SF / 2001 / Microbial biosensors / Biosens. Bioelectron 16:337~353
Faruque SM / 2012 / Phage-bacterial interactions in the evolution of toxigenic Vibrio cholerae / Virulence 3:556~565
Lazcka O / 2007 / Pathogen detection: a perspective of traditional methods and biosensors / Biosens. Bioelectron 22:1205~1217
Dwivedi HP / 2011 / Detection of pathogens in foods:the current state-of-the-art and future directions / Crit. Rev. Microbiol 37:40~63
D'Souza SF / 2001 / Microbial biosensors / Biosens. Bioelectron 16:337~353
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