Screening of Anti-Biofilm Compounds from Marine-Derived Fungi and the Effects of Secalonic Acid D on Staphylococcus aureus Biofilm

Jie Wang; Xu-Hua Nong; Xiaoyong Zhang; Xin-Ya Xu; Muhammad Amin; Shu-Hua Qi

doi:10.4014/jmb.1609.09053

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자료유형: 학술저널

저자정보: Jie Wang (Chinese Academy of Sciences) Xu-Hua Nong (Chinese Academy of Sciences) Xiaoyong Zhang (Chinese Academy of Sciences) Xin-Ya Xu (Chinese Academy of Sciences) Muhammad Amin (Chinese Academy of Sciences) Shu-Hua Qi (Chinese Academy of Sciences)

저널정보: 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제6호

발행연도: 2017.6

수록면: 1,078 - 1,089 (12page)

DOI: 10.4014/jmb.1609.09053

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Biofilm formation of Staphylococcus aureus is one of its mechanisms of drug resistance. Antibiofilm screening of 106 compounds from marine-derived fungi displayed that 12 compounds inhibited S. aureus biofilm formation by >50% at the concentration of 100 μg/ml, and only secalonic acid D (SAD) and B inhibited by >90% at 6.25 μg/ml without inhibiting cell growth after 24-h incubation. Meanwhile, it was found that the double bond between C-1 and C-10 of citrinin derivatives and the C-C connection position of two chromone monomers may be important for their anti-biofilm activities. Moreover, SAD slightly facilitated biofilm eradication and influenced its architecture. Furthermore, SAD slowed the cell growth rate in the preceding 18-h incubation and differentially regulated transcriptional expression of several genes, such as agr, isaA, icaA, and icaD, associated with biofilm formation in planktonic and biofilm cells, which may be the reason for the anti-biofilm activity of SAD. Finally, SAD acted synergistically against S. aureus growth and biofilm formation with other antibiotics. These findings indicated that various natural products from marine-derived fungi, such as SAD, could be used as a potential biofilm inhibitor against S. aureus.

#Anti-biofilm activity #Staphylococcus aureus #marine natural product #secalonic acid D #mechanism of action

참고문헌 (46)

참고문헌 신청

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