Anaerobic Ammonium-Oxidizing Bacteria in Cow Manure Composting

Tingting Wang; Lijun Cheng; Wenhao Zhang; Xiuhong Xu; Qingxin Meng; Xuewei Sun; Huajing Liu; Hongtao Li; Yu Sun

doi:10.4014/jmb.1702.02065

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자료유형: 학술저널

저자정보: Tingting Wang (Northeast Agricultural University) Lijun Cheng (Northeast Agricultural University) Wenhao Zhang (Northeast Agricultural University) Xiuhong Xu (Northeast Agricultural University) Qingxin Meng (Northeast Agricultural University) Xuewei Sun (Northeast Agricultural University) Huajing Liu (Northeast Agricultural University) Hongtao Li (Northeast Agricultural University) Yu Sun (Northeast Agricultural University)

저널정보: 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제7호

발행연도: 2017.7

수록면: 1,288 - 1,299 (12page)

DOI: 10.4014/jmb.1702.02065

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Composting is widely used to transform waste into valuable agricultural organic fertilizer. Anaerobic ammonium-oxidizing (anammox) bacteria play an important role in the global nitrogen cycle, but their role in composting remains poorly understood. In the present study, the community structure, diversity, and abundance of anammox bacteria were analyzed using cloning and sequencing methods by targeting the 16S rRNA gene and the hydrazine oxidase gene (hzo) in samples isolated from compost produced from cow manure and rice straw. A total of 25 operational taxonomic units were classified based on 16S rRNA gene clone libraries, and 14 operational taxonomic units were classified based on hzo gene clone libraries. The phylogenetic tree analysis of the 16S rRNA gene and deduced HZO protein sequences from the corresponding encoding genes indicated that the majority of the obtained clones were related to the known anammox bacteria Candidatus “Brocadia,” Candidatus “Kuenenia,” and Candidatus “Scalindua.” The abundances of anammox bacteria were determined by quantitative PCR, and between 2.13 × 105 and 1.15 × 106 16S rRNA gene copies per gram of compost were found. This study provides the first demonstration of the existence of anammox bacteria with limited diversity in cow manure composting

#Anammox bacteria #compost #16S rRNA gene #hzo gene #nitrogen cycle

참고문헌 (45)

참고문헌 신청

Jetten MS / 2009 / Biochemistry and molecular biology of anammox bacteria / Crit. Rev. Biochem. Mol 44:65~84

Mulder A / 1995 / Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor / FEMS Microbiol. Ecol 16:177~188

Kartal B / 2008 / Candidatus ‘Brocadia fulgida’: an autofluorescent anaerobic ammonium oxidizing bacterium / FEMS Microbiol. Ecol 63:46~55

Schmidt I / 2002 / Aerobic and anaerobic ammonia oxidizing bacteria - competitors or natural partners? / FEMS Microbiol. Ecol 39:175~181

Schmid M / 2003 / Candidatus 'Scalindua brodae', sp. nov., Candidatus 'Scalindua wagneri', sp. nov., the two new species of anaerobic ammonium oxidizing bacteria / Syst. Appl. Microbiol 26:529~538

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