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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 1,223 - 1,230 (8page)
- DOI
- 10.1007/s13258-021-01151-7
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초록· 키워드
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Background Vacuolating cytotoxin (VacA) is an important virulence factor of Helicobacter pylori (H. pylori). It was previously believed that VacA can trigger the cascade of apoptosis on mitochondria to lead to cell apoptosis. Recently, it was found that VacA can induce autophagy. However, the molecular mechanism by which VacA induces autophagy is largely unknown.
Objective We aimed to explore the molecular mechanism of autophagy induced by H. pylori in gastric cancer cells and the efect of autophagy on the survival of gastric cancer cells.
Methods The autophagy of human gastric cancer cell line SGC7901 was detected by Western blot and RT-PCR in the treatment of VacA protein of H. pylori. The relationship between autophagy and reactive oxygen species (ROS) in the proliferation of gastric cancer cells were studied by gene expression silences (siRNA) and CM-H2DCFDA (DCF) staining.
Results The results showed that VacA protein secreted by H. pylori in the supernatant stimulated autophagy in SGC7901 cells. After VacA protein treatment, the mRNA expressions of BECN1, ATG7 and PIK3C3, were up-regulated. ATG7 silencing by siRNA inhibited VacA-induced autophagy. Furthermore, our data demonstrated that VacA protein increased ROS levels. Addition of the antioxidant N-acetyl-l-cysteine (NAC) suppressed the levels of ROS, leading to inhibition of autophagy.
Conclusions H. pylori VacA is a key toxin that induces autophagy by increased ROS levels. And our fndings demonstrated that VacA signifcantly inhibited proliferation in SGC7901 cells.
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등록된 정보가 없습니다.
참고문헌 (32)
참고문헌 신청
Bou DP / 2014 / A conserved role for p48 homologs in protecting dopaminergic neurons from oxidative stress / PLoS Genet 10(10):e1004718
Bronte-Tinkew DM / 2009 / Helicobacter pylori cytotoxin-associated gene A activates the signal transducer and activator of transcription 3 pathway in vitro and in vivo / Cancer Res 69(2):632~639
Butcher LD / 2017 / Oxidative stress resulting from Helicobacter pylori infection contributes to gastric carcinogenesis / Cell Mol Gastroenterol Hepatol 3(3):316~322
Calvino FM / 2010 / H. pylori and mitochondrial changes in epithelial cells. The role of oxidative stress / Rev Esp Enferm Dig 102(1):41~50
Circu ML / 2010 / Reactive oxygen species, cellular redox systems, and apoptosis / Free Radic Biol Med 48(6):749~762
Bronte-Tinkew DM / 2009 / Helicobacter pylori cytotoxin-associated gene A activates the signal transducer and activator of transcription 3 pathway in vitro and in vivo / Cancer Res 69(2):632~639
Butcher LD / 2017 / Oxidative stress resulting from Helicobacter pylori infection contributes to gastric carcinogenesis / Cell Mol Gastroenterol Hepatol 3(3):316~322
Calvino FM / 2010 / H. pylori and mitochondrial changes in epithelial cells. The role of oxidative stress / Rev Esp Enferm Dig 102(1):41~50
Circu ML / 2010 / Reactive oxygen species, cellular redox systems, and apoptosis / Free Radic Biol Med 48(6):749~762
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