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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 362 - 370 (9page)
- DOI
- 10.1007/s13206-021-00035-y
이용수
초록· 키워드
오류제보하기
In human pregnancy, successful invasion/migration of trophoblast cells under controlled oxygen pressure regulates the fetus development, while a defective invasion leads to obstetric complications including miscarriage, pre-eclampsia, placenta abruption, and intrauterine death. However, the underlying mechanism of defective cell invasion by hypoxia is still unknown.
Conventional systems employed to study this cell invasion/migration have several limitations such as non-tunability, and absence of physiological conditions. Herein, we reported a polydimethylsiloxane (PDMS) based simple interdigitated disk platform to study the cell invasion/migration under hypoxic (0.5% O 2 ) and normoxic (21% O 2 ) conditions. Human trophoblast cells (HTR-8/SVneo) and human umbilical vein endothelial cells (HUVEC) were seeded on the interdigitated disk separated by an extracellular matrix (ECM) layer. The trophoblast invasion to the HUVEC side was analyzed and compared under both hypoxic and normoxic conditions. Furthermore, the secretion of biological factors, hypoxic induced factor 1-alpha (HIF-1α), and matrix metalloproteinase (MMP) -2 and 9, involved in trophoblast invasion were also analyzed using western blot and zymography. The results showed that under long-term exposure to a hypoxic environment, cells secrete a high amount of MMP-9, which is responsible for ECM breakdown and enhanced trophoblast invasion. Hence, the long-term hypoxic exposure improves the invasion mechanism of trophoblast. Therefore, we suggest that a PDMS-based interdigitated device could be a useful tool to understand the mechanisms of cell-to-cell interaction as well as the invasion of trophoblast by oxygen pressure.
목차
등록된 정보가 없습니다.
참고문헌 (39)
참고문헌 신청
Margherita Y. Turco / 2019 / Development of the human placenta / Development 146(22)
Margherita Y. Turco / 2018 / Trophoblast organoids as a model for maternal–fetal interactions during human placentation / Nature 564(7735):263~267
조혜란 / 2019 / Recent Clinical Trials in Adipose-derived Stem Cell Mediated Osteoarthritis Treatment / Biotechnology and Bioprocess Engineering 24(6):839~853
G.J. Burton / 2012 / Review: The placenta and developmental programming: Balancing fetal nutrient demands with maternal resource allocation / Placenta 33:S23~S27
Neil M. Gude / 2004 / Growth and function of the normal human placenta / Thrombosis Research 114(5-6):397~407
Margherita Y. Turco / 2018 / Trophoblast organoids as a model for maternal–fetal interactions during human placentation / Nature 564(7735):263~267
조혜란 / 2019 / Recent Clinical Trials in Adipose-derived Stem Cell Mediated Osteoarthritis Treatment / Biotechnology and Bioprocess Engineering 24(6):839~853
G.J. Burton / 2012 / Review: The placenta and developmental programming: Balancing fetal nutrient demands with maternal resource allocation / Placenta 33:S23~S27
Neil M. Gude / 2004 / Growth and function of the normal human placenta / Thrombosis Research 114(5-6):397~407
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