지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Bis-(3',5')-cyclic-dimeric-guanosine monophosphate (c-di-GMP), a bacterial secondary messenger, is a crucial regulator ofcollective versus individual behavior in bacteria. Diguanylate cyclases (DGCs) are essential for the synthesis of c-di-GMP.
To elucidate the structural features of DGCs, we initiated the crystallization of a putative DGC (Tm0107) from Thermotogamaritima. Tm0107 protein was overexpressed in Escherichia coli and crystallized at 295 K using PEG 3000 as a precipitant.
Crystals of Tm0107 protein diffracted to 2.1 A resolution and belong to a Tetragonal space group P43212, with unit cellparameters of a = 61.3 A, b = 61.3 A and c = 101.1 A. The crystallization of selenomethionine-substituted protein is inprogress to solve the crystal structure of Tm0107 protein.
목차
등록된 정보가 없습니다.
참고문헌 (13)
참고문헌 신청
Chan, C / 2004 / Structural basis of activity and allosteric control of diguanylate cyclase / Proc Natl Acad Sci USA 101:17084~17089
Christen, B / 2006 / Allosteric control of cyclic di-GMP signaling / J Biol Chem 281:32015~32024
Hengge, R / 2009 / Principles of c-di-GMP signalling in bacteria / Nat Rev Microbiol 7:263~273
Jenal, U / 2004 / Cyclic di-guanosine-monophosphate comes of age: a novel secondary messenger involved in modulating cell surface structures in bacteria? / Curr Opin Microbiol 7:185~191
Laemmli, U. K / 1970 / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 / Nature 227:680~685
Christen, B / 2006 / Allosteric control of cyclic di-GMP signaling / J Biol Chem 281:32015~32024
Hengge, R / 2009 / Principles of c-di-GMP signalling in bacteria / Nat Rev Microbiol 7:263~273
Jenal, U / 2004 / Cyclic di-guanosine-monophosphate comes of age: a novel secondary messenger involved in modulating cell surface structures in bacteria? / Curr Opin Microbiol 7:185~191
Laemmli, U. K / 1970 / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 / Nature 227:680~685
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