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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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CRISPR/Cas system is an RNA-mediated prokaryotic adaptive immune system against mobile genetic elements. Amongthree CRISPR/Cas systems, type I system has two functional units in removing nucleic acids, Cascade and Cas3. TypeIB Cascade has been analyzed to have components of Csh1, Csh2, Cas4, and Cas5, whose structural and functionalinformation is not available. To provide a structural background for the type IB Cascade, the preliminary structural study ofthe Csh2 from Thermobaculum terrenum is reported here. The purified recombinant protein was successfully crystallizedusing the precipitant solution composed of 10% (w/v) Polyethylene Glycol 3350, 2% (v/v) Tacsimate at pH 5.0, and 100mM Sodium Citrate tribasic dihydrate at pH 5.6. Diffraction data were collected to a resolution of 1.93 A using synchrotronradiation. The crystal belongs to the primitive triclinic P1 space group, with unit cell dimensions a = 31.04 A, b = 54.65 A,c = 96.46 A, α = 73.6 º, β = 88.4 º and γ = 89.8 º. With two molecules in the asymmetric unit, the crystal volume per unitprotein weight corresponds with 2.29 A3·Da-1, resulting in 46% solvent.
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참고문헌 (38)
참고문헌 신청
Amitai, G / 2016 / CRISPR-Cas adaptation : insights into the mechanism of action / Nat Rev Microbiol 14:67~76
Arslan, Z / 2014 / Detection and characterization of spacer integration intermediates in type IE CRISPR–Cas system / Nucleic Acids Res 42:7884~7893
Aslanidis, C / 1990 / Ligation-independent cloning of PCR products(LIC-PCR) / Nucleic Acids Res 18:6069~6074
Barrangou, R / 2007 / CRISPR provides acquired resistance against viruses in prokaryotes / Science 315:1709~1712
Barrangou, R / 2014 / CRISPR-Cas systems : prokaryotes upgrade to adaptive immunity / Molecular cell 54:234~244
Arslan, Z / 2014 / Detection and characterization of spacer integration intermediates in type IE CRISPR–Cas system / Nucleic Acids Res 42:7884~7893
Aslanidis, C / 1990 / Ligation-independent cloning of PCR products(LIC-PCR) / Nucleic Acids Res 18:6069~6074
Barrangou, R / 2007 / CRISPR provides acquired resistance against viruses in prokaryotes / Science 315:1709~1712
Barrangou, R / 2014 / CRISPR-Cas systems : prokaryotes upgrade to adaptive immunity / Molecular cell 54:234~244
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