지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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A solid-phase competition enzyme-linked immunosorbent assay (ELISA), recombinant VP2 (rVP2) protein, and monoclonal antibody (mAb) were developed for the specific and sensitive detection of porcine parvovirus (PPV) antibodies in pig sera. A total of 1,544 sera samples were collected from breeding pig farms located in the Gyeongsangbuk-do Province in the Republic of Korea.
The optimal operating conditions of SC-ELISA were as follows. The concentration of rVP2 proteins coated on the wells was 4 μg/mL, the swine sera were diluted 1:2, and the HRP-conjugated PPV VP2 mAb (9A8 clone) was used at 500 ng/mL. These results suggest that the SC-rVP-ELISA assay may be a valuable alternative to the current diagnostic tools used to detect PPV-specific monoclonal antibodies and broadly monitor PPV infections in domestic pigs at different breeding stages.
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참고문헌 (27)
참고문헌 신청
Antonis AFG / 2006 / A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure / Vaccine 24(26):5481~5490
Cohen J / 1960 / A Coefficient of Agreement for Nominal Scales / Educational and Psychological Measurement 20(1):37~46
Drolet R / 2002 / Infectious agents identified in pigs with multifocal interstitial nephritis at slaughter / Veterinary Record 150(5):139~143
Ellis J / 2004 / Porcine circovirus-2 and concurrent infections in the field / Veterinary Microbiology 98(2):159~163
Hajian-Tilaki K / 2013 / Receiver Operating Characteristic(ROC)Curve Analysis for Medical Diagnostic Test Evaluation / Caspian Journal of Internal Medicine 4(2):627~635
Cohen J / 1960 / A Coefficient of Agreement for Nominal Scales / Educational and Psychological Measurement 20(1):37~46
Drolet R / 2002 / Infectious agents identified in pigs with multifocal interstitial nephritis at slaughter / Veterinary Record 150(5):139~143
Ellis J / 2004 / Porcine circovirus-2 and concurrent infections in the field / Veterinary Microbiology 98(2):159~163
Hajian-Tilaki K / 2013 / Receiver Operating Characteristic(ROC)Curve Analysis for Medical Diagnostic Test Evaluation / Caspian Journal of Internal Medicine 4(2):627~635
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