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Environmental mastitis has increased particularly in well-managed or low somatic cell count herds that have successfully controlled contagious pathogens. Major pathogens of environmental mastitis are Escherichia coli (E. coli) and Streptococcus uberis. The present study was conducted to investigate the isolation and antibiotic susceptibility of 406 (21.8%) gram-negative bacteria isolated mastitis milk from 1,865 quaters of 241 Korean dairy farms from 2001 to 2004. Prevalence of major gram-negative bacteria isolated from mastitis milk were E. coli (22.7%) and Enterobacter spp. (16.3%) in coliforms and Pseudomoas spp. (10.3%) and Serratia spp. (7.9%) in non-coliforms. The results on antibiotic susceptibility by agar diffusion test against these pathogens were 86.7% in piperaciliin, 94.6% in cefepime, 85.5% in amikacin, 87.7% in gentamicin and so on. In contrast, the susceptibility against ampicillin (41.9%), cephalothin (9.9%), streptomycin (39.9%) and tetracycline (46.7%) appeared to be below 50%. Gram-negative bacteria showed the high minimal inhibitory concentration on cephalothin (96.1%), streptomycin (99.5%) and tetracycline (96.8%). According to year, distribution of high 256~64 μg/ml on cephalothin get increased, but the others are different. These findings demonstrate that major gram-negative bacteria were E. coli and Enterobacter spp. isolates, and often encountered the diverse antibiotic resistant patterns.

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UCI(KEPA) : I410-ECN-0101-2023-528-001379598