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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.3
- 수록면
- 242 - 249 (8page)
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A balance between production and degradation of reactive oxygen species (ROS) is critical for maintaining cellular homeostasis. Increased levels of ROS during oxidative stress are associated with disease conditions. Antioxidant enzymes, such as extracellular superoxide dismutase (EC-SOD), in the extracellular matrix (ECM) neutralize the toxicity of superoxide. Recent studies have emphasized the importance of EC-SOD in protecting the brain, lungs, and other tissues from oxidative stress. Therefore, EC-SOD would be an excellent therapeutic drug for treatment of diseases caused by oxidative stress. We cloned both the full length (residues 1-240) and truncated (residues 19-240) forms of human EC-SOD (hEC-SOD) into the donor plasmid pFastBacHTb. After transposition, the bacmid was transfected into the Sf9-baculovirus expression system and the expressed hEC-SOD purified using FLAG-tag. Western blot analysis revealed that hEC-SOD is present both as a monomer (33 kDa) and a dimer (66 kDa), as detected by the FLAG antibody. A water-soluble tetrazolium (WST-1) assay showed that both full length and truncated hEC-SOD proteins were enzymatically active. We showed that a potent superoxide dismutase inhibitor, diethyldithio-carbamate (DDC), inhibits hEC-SOD activity.
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참고문헌 (33)
참고문헌 신청
Antonyuk, S. V. / 2009 / The structure of human extracellular copper-zinc superoxide dismutase at 1. 7 Å resolution : insights into heparin and collagen binding / J. Mol. Biol 388:310~326
Arnelle, D. R. / 1997 / Diethyl dithiocarbamate-induced decomposition of S-nitrosothiols / Nitric Oxide 1:56~64
Bae, J. Y. / 2013 / Cu/Zn incorporation during purification of soluble human EC-SOD from E. coli stabilizes proper disulfide bond formation / Appl. Biochem. Biotechnol 169:1633~1647
Beyer, W. / 1991 / Superoxide dismutases / Prog. Nucl. Acid Res. Mol. Biol 40:221~253
Bowler, R. P. / 2002 / Role of extracellular superoxide dismutase in bleomycin-induced pulmonary fibrosis / Am. J. Physiol. Lung Cell Mol. Physiol 282:719~726
Arnelle, D. R. / 1997 / Diethyl dithiocarbamate-induced decomposition of S-nitrosothiols / Nitric Oxide 1:56~64
Bae, J. Y. / 2013 / Cu/Zn incorporation during purification of soluble human EC-SOD from E. coli stabilizes proper disulfide bond formation / Appl. Biochem. Biotechnol 169:1633~1647
Beyer, W. / 1991 / Superoxide dismutases / Prog. Nucl. Acid Res. Mol. Biol 40:221~253
Bowler, R. P. / 2002 / Role of extracellular superoxide dismutase in bleomycin-induced pulmonary fibrosis / Am. J. Physiol. Lung Cell Mol. Physiol 282:719~726
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