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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 512 - 521 (10page)
- DOI
- 10.4014/mbl.1609.09003
이용수
초록· 키워드
오류제보하기
A bacterial strain was isolated from a soil sample collected on Jeju Island, Korea. The strain, designated WJ-2, exhibited a high xylanase activity, whereas cellulase activity was not detected. The 16S rRNA gene sequence of WJ-2 was highly similar to type strains of the genus Streptomyces. A neighbor-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain WJ-2 is phylogenetically related to Streptomyces atrovirens. Furthermore, DNA-DNA hybridization analysis confirmed that strain WJ-2 is a novel subspecies of Streptomyces atrovirens. The genomic DNA G+C content was 73.98 mol% and the major fatty acid present was anteiso-C15:0 (36.19%). The growth and xylanase production of strain WJ-2 were significantly enhanced by using soytone and xylan as nitrogen and carbon sources, respectively. Crude enzyme preparations from the culture broth of strain WJ-2 exhibited maximal total xylanase activities at pH 7.0 and 55℃. Thin-layer chromatography analysis revealed that the crude enzyme degrades beechwood xylan to yield xylobiose and xylotriose as the principal hydrolyzed end products.
목차
등록된 정보가 없습니다.
참고문헌 (38)
참고문헌 신청
Achary AA / 2009 / Value addition to corncob: Production and characterization of xylo-oligosaccharides from alkali pretreated lignin-saccharide complex using Aspergillus oryzae MTCC5154 / Bioresour. Technol 100:991~995
Al-Bari MAA / 2005 / Streptomyces bangladeshensis sp. nov., isolated from soil, which produces bis-(2-ethylhexyl) phthalate / Int. J. Syst. Evol. Microbiol 55:1973~1977
Altschul SF / 1990 / Gapped BLAST and PSI-BLAST: a new generation of protein database search programs / Nucleic Acids Res 25:3389~3402
Bajaj BK / 2010 / Production of xylanase from an alkalitolerant Streptomyces sp. 7b under solid-state fermentation, its purification, and characterization / Appl. Biochem. Biotechnol 162:1804~1818
Baker GC / 2003 / Review and re-analysis of domain-specific 16S primers / J. Microbiol. Methods 55:541~555
Al-Bari MAA / 2005 / Streptomyces bangladeshensis sp. nov., isolated from soil, which produces bis-(2-ethylhexyl) phthalate / Int. J. Syst. Evol. Microbiol 55:1973~1977
Altschul SF / 1990 / Gapped BLAST and PSI-BLAST: a new generation of protein database search programs / Nucleic Acids Res 25:3389~3402
Bajaj BK / 2010 / Production of xylanase from an alkalitolerant Streptomyces sp. 7b under solid-state fermentation, its purification, and characterization / Appl. Biochem. Biotechnol 162:1804~1818
Baker GC / 2003 / Review and re-analysis of domain-specific 16S primers / J. Microbiol. Methods 55:541~555
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