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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제12호
- 발행연도
- 2016.12
- 수록면
- 2,066 - 2,075 (10page)
- DOI
- 10.4014/jmb.1605.05057
이용수
초록· 키워드
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In this study, an enhanced lipid-producing mutant strain of the microalga Chlamydomonas reinhardtii was developed by gamma irradiation. To induce the mutation, C. reinhardtii was gamma irradiated at a dose of 400 Gy. After irradiation, the surviving cells were stained with Nile red. The mutant (Cr-4013) accumulating 20% more lipid than the wild type was selected. Thin-layer chromatography revealed the triglyceride and free fatty acid contents to be markedly increased in Cr-4013. The major fatty acids identified were palmitic acid, oleic acid, linoleic acid, and linolenic acid. Random amplified polymeric DNA analysis showed partial genetic modifications in Cr-4013. To ascertain the changes of protein expression in the mutant strain, two-dimensional electrophoresis was conducted. These results showed that gamma radiation could be used for the development of efficient microalgal strains for lipid production.
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참고문헌 (40)
참고문헌 신청
Ahloowalia BS / 2001 / Induced mutations –a new paradigm in plant breeding / Euphytica 118:167~173
Ahmad AL / 2011 / Microalgae as a sustainable energy source for biodiesel production: a review / Renew. Sustain. Energy Rev 15:584~593
Amaro HM / 2011 / Advances and perspectives in using microalgae to produce biodiesel / Appl. Energy 88:3402~3410
Beer LL / 2009 / Engineering algae for biohydrogen and biofuel production / Curr. Opin. Biotechnol 20:264~271
Berberoglu H / 2009 / Radiation characteristics of Botryococcus braunii, Chlorococcum littorale, and Chlorella sp. used for CO2 fixation and biofuel production / J. Quant. Spectrosc. Radiat. Transf 110:1879~1893
Ahmad AL / 2011 / Microalgae as a sustainable energy source for biodiesel production: a review / Renew. Sustain. Energy Rev 15:584~593
Amaro HM / 2011 / Advances and perspectives in using microalgae to produce biodiesel / Appl. Energy 88:3402~3410
Beer LL / 2009 / Engineering algae for biohydrogen and biofuel production / Curr. Opin. Biotechnol 20:264~271
Berberoglu H / 2009 / Radiation characteristics of Botryococcus braunii, Chlorococcum littorale, and Chlorella sp. used for CO2 fixation and biofuel production / J. Quant. Spectrosc. Radiat. Transf 110:1879~1893
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