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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.6
- 수록면
- 109 - 118 (10page)
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초록· 키워드
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The gene encoding an esterase enzyme was cloned from a metagenomic library of cow rumen bacteria. The esterase gene (est2R) was 2,120 bp in length, encoding a protein of 516 amino acid residues with a calculated molecular weight of 57,286 Da. The molecular weight of the enzyme was estimated to be 57,000 Da by SDS-PAGE. Est2R shared 35.6% amino acid identity with esterase (CAH19079) of uncultured prokaryote. The Est2R was most active at 20-40°C, and showed optimum at 30°C and pH 8.0. The most activity of Est2R for the different chain length of p-nitrophenyl ester group as substrate was p-nitrophenyl acetate. Moreover, the enzyme was found to be most active without organic solvent, followed by 98% active with ethanol, and the enzyme activity was highly affected by the acetonitrile. The enzyme was significantly inhibited by Zn2+ but stimulated by Ca2+. So, novel esterase gene est2R is likely to obtain from cow rumen metagenome and supposed to use for industrial purpose.
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참고문헌 (28)
참고문헌 신청
Bradford, M. M / 1976 / A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72:248~254
Choi, Y. J / 2004 / Charaterization and heterologous gene expression of a novel esterase from Lactobacillus casei CL96 / Appl. Environ. Microbiol 70:3213~3221
Clarke, D. G / 1970 / Studies on rumen metabolism. VI. In vitro hydrolysis of triglyceride and isolation of a lipolytic fraction / J. Sci. Food Agr 21:446~452
Daniel, R / 2004 / The soil metagenome-a rich resource for the discovery of novel natural products / Curr. Opin. Biotechnol 15:199~204
Elend, C / 2006 / Isolation and biochemical characterization of two novel metagenome-derived esterases / Appl. Environ. Microbiol 72:3637~3645
Choi, Y. J / 2004 / Charaterization and heterologous gene expression of a novel esterase from Lactobacillus casei CL96 / Appl. Environ. Microbiol 70:3213~3221
Clarke, D. G / 1970 / Studies on rumen metabolism. VI. In vitro hydrolysis of triglyceride and isolation of a lipolytic fraction / J. Sci. Food Agr 21:446~452
Daniel, R / 2004 / The soil metagenome-a rich resource for the discovery of novel natural products / Curr. Opin. Biotechnol 15:199~204
Elend, C / 2006 / Isolation and biochemical characterization of two novel metagenome-derived esterases / Appl. Environ. Microbiol 72:3637~3645
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