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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Breast cancer is the most common cancer among women worldwide. There is an emerging interest in protein expressionprofiling with the aim of identifying novel diagnostic markers and therapeutic targets in breast cancer. We analyzed breastcancer tissues by using the cICAT (cleavable isotope-coded affinity tag) labeling approach and tandem mass spectrometry.
Breast cancer and matched normal tissues were fractionated by ultracentrifugation to enrich membrane proteins, andcICAT labeled proteins were separated by SDS-PAGE. A total of 364 proteins were identified and quantified. Among theproteins that showed >1.5 fold difference between normal and cancer (36.6%), five (RPN1, ERp29, Trop2, PrP and PIGR)were selected for confirmation. Western blot on 10 pairs of normal and cancer tissues revealed that the expression ofRPN1 and ERp29 were increased in breast cancer tissue, while Trop2, PrP and PIGR were decreased. Those Western blotresults were highly consistent with cICAT results. Higher level of RPN1 and ERp29 and lower level of Trop2 in breast cancercell lines compared to normal breast cell lines were observed. The result supports reliability of our SDS-PAGE based ICATmethod in quantifying cancer tissue proteome.
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참고문헌 (28)
참고문헌 신청
Bagshaw, R. D. / 2005 / A proteomic analysis of lysosomal integral membrane proteins reveals the diverse composition of the organelle / Mol Cell Proteomics 4:133~143
Bambang, I. F. / 2009 / Cytokeratin 19 regulates endoplasmic reticulum stress and inhibits ERp29 expression via p38 MAPK/XBP-1 signaling in breast cancer cells / Exp Cell Res 315:1964~1974
Berntsson, J. / 2014 / Expression and prognostic significance of the polymeric immunoglobulin receptor in epithelial ovarian cancer / J Ovarian Res 7:26
Chatterjee, S. K. / 2005 / Cancer biomarkers : knowing the present and predicting the future / Future Oncol 1:37~50
F, D. I. G. / 2010 / A simple and effective method to analyze membrane proteins by SDS-PAGE and MALDI mass spectrometry / Anticancer Res 30:1121~1129
Bambang, I. F. / 2009 / Cytokeratin 19 regulates endoplasmic reticulum stress and inhibits ERp29 expression via p38 MAPK/XBP-1 signaling in breast cancer cells / Exp Cell Res 315:1964~1974
Berntsson, J. / 2014 / Expression and prognostic significance of the polymeric immunoglobulin receptor in epithelial ovarian cancer / J Ovarian Res 7:26
Chatterjee, S. K. / 2005 / Cancer biomarkers : knowing the present and predicting the future / Future Oncol 1:37~50
F, D. I. G. / 2010 / A simple and effective method to analyze membrane proteins by SDS-PAGE and MALDI mass spectrometry / Anticancer Res 30:1121~1129
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