지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.6
- 수록면
- 201 - 208 (8page)
이용수
초록· 키워드
오류제보하기
The main areas for field-grown vegetable production in Iran were surveyed during the years of 2012.2014 to determine the occurrence of begomoviruses infecting these crops. A total of 787 leaf samples were collected from vegetables and some other host plants showing virus-like symptoms and tested by an enzymelinked immunosorbent assay (ELISA) using polyclonal antibodies produced against Tomato yellow leaf curl virus (TYLCV). According to the ELISA results, 81 samples (10.3%) positively reacted with the virus antibodies. Begomovirus infections were confirmed by polymerase chain reaction (PCR) using previously described TYLCV-specific primer pair TYLCV-Sar/ TYLCV-Isr or universal primer pair Begomo-F/ Begomo-R. The PCR tests using the primer pair TYLCV-Sar/TYLCV-Isr resulted in the amplification of the expected fragments of ca. 0.67-kb in size for ELISA-positive samples tested from alfalfa, pepper, spinach and tomato plants, confirming the presence of TYLCV. For one melon sample, having a week reaction in ELISA and no reaction in PCR using TYLCVspecific primers, the PCR reaction using the primer pair Begomo-F/Begomo-R resulted in the amplification fragments of the expected size of ca. 2.8 kb. The nucleotide sequences of the DNA amplicons derived from the isolate, Kz-Me198, were determined and compared with other sequences available in GenBank. BLASTN analysis confirmed the begomovirus infection of the sample and showed 99% identities with Tomato leaf curl New Delhi virus (ToLCNDV); phylogenetic analysis supported the results of the database searches. This study reports the natural occurrence of TYLCV in different hosts in Iran. Our results also reveal the emergence of ToLCNDV in Iranian cucurbit crops.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
A Simple and Reliable Molecular Detection Method for Tomato yellow leaf curl virus in Solanum lycopersicum without DNA Extraction
Research in Plant Disease(식물병연구)
2015 .09
-
Tomato Yellow Leaf Curl Virus Infection in a Monocotyledonous Weed (Eleusine indica)
The Plant Pathology Journal
2021 .12
-
Comparative Analyses of Tomato yellow leaf curl virus C4 Protein-Interacting Host Proteins in Healthy and Infected Tomato Tissues
The Plant Pathology Journal
2016 .10
-
Seed Transmission of Tomato yellow leaf curl virus in White Soybean (Glycine max)
The Plant Pathology Journal
2017 .08
-
Twindemic Threats of Weeds Coinfected with Tomato Yellow Leaf Curl Virus and Tomato Spotted Wilt Virus as Viral Reservoirs in Tomato Greenhouses
The Plant Pathology Journal
2024 .06
-
Antagonism of tomato spotted wilt virus against tomato yellow leaf curl virus in Nicotiana benthamiana detected by transcriptome analysis
Genes & Genomics
2023 .01
-
The Insidious Threat: Assessing the Dangers and Spread of Tomato Leaf Curl New Delhi Virus
The Plant Pathology Journal
2025 .02
-
토마토황화잎말림바이러스병에 대한 저항성 품종과 항바이러스 활성 물질 3종의 효과 검증
Research in Plant Disease(식물병연구)
2022 .06
-
Physiology and Gene Expression Analysis of Tomato (Solanum lycopersicum L.) Exposed to Combined-Virus and Drought Stresses
The Plant Pathology Journal
2023 .10
-
경기도 광주 여름재배 시설토마토의 가루이 매개 바이러스 TYLCV, ToCV 발생현황
Research in Plant Disease(식물병연구)
2022 .03
-
국내의 토마토 주요 바이러스 진단을 위한 역전사중합반응법용 프라이머 세트
Research in Plant Disease(식물병연구)
2017 .06
-
토마토품종의 토마토황화잎말림바이러스병에 대한 저항성 평가
Research in Plant Disease(식물병연구)
2016 .12
-
Development of a Multiplex Polymerase Chain Reaction Assay for Detecting Five Previously Unreported Papaya Viruses for Quarantine Purposes in Korea
Research in Plant Disease(식물병연구)
2024 .09
-
Development of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for the Simultaneous Detection of Three Viruses in Leguminous Plants
Research in Plant Disease(식물병연구)
2018 .12
-
A New Distinct Clade for Iranian Tomato spotted wilt virus Isolates Based on the Polymerase, Nucleocapsid, and Non-structural Genes
The Plant Pathology Journal
2018 .12
-
Development of a Multiplex RT-PCR for the Simultaneous Detection of Three Viruses in Cherry Plants
Research in Plant Disease(식물병연구)
2018 .09
-
Characterization of a Begomovirus-Betasatellite Complex, Producing Defective Molecules in Spinach (Spinacia oleracea L.), a New Host for Begomovirus and Betasatellite Complex in Pakistan
The Plant Pathology Journal
2017 .10
-
Simultaneous Detection of Four Foodborne Viruses in Food Samples Using a One-Step Multiplex Reverse Transcription PCR
Journal of Microbiology and Biotechnology
2018 .01
-
한국과 중국 채집 담배가루이 지역계통의 B, Q biotype 분포 및 살충제 약제반응, 토마토황화잎말림바이러스 보독율 비교
환경생물
2020 .01
-
국내 맥류에 발생하는 바이러스병 동시진단 방법
Research in Plant Disease(식물병연구)
2017 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0102-2023-481-001835388