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자료유형
학술저널
저자정보
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한국환경과학회 한국환경과학회지 한국환경과학회지 제25권 제4호
발행연도
2016.4
수록면
473 - 481 (9page)

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We examined the effects of ocean acidification (OA) and eutrophication on the physiology of a red alga, Gracilariopsis chorda, using specimens collected at Wando Island, Korea, in July of 2015. The samples were transported to a laboratory and placed on growth media for treatments involving low or high levels of ammonium (4 μM or 60 μM NH<sub>4</sub>+) and low or high pH (7.5 or 8.2). The control treatment used filtered seawater (pH 8.2 and 4 μM NH<sub>4</sub>+). All experiments were conducted at 20°Cand under a lighting intensity of 80 μmol photons m<sup>-2</sup> s<sup>-1</sup>, with or without an injection of CO<sub>2</sub> (pH 7.5). In addition, we calculated rates of respiration under darkness, at a pH of 7.5 and 60 μM NH<sub>4</sub>+. Fluctuations in pH as well as the evolution of photosynthetic oxygen and NH<sub>4</sub>+ uptake rates were monitored for 6 h. The greatest increase in pH levels, from 7.50 to 8.65, occurred in response to 60 μM NH<sub>4</sub>+, whereas the largest decrease, from 7.50 to 7.42, was associated with elevated respiration rates. At a pH of 7.5, rates of oxygen evolution were higher (236% saturation) for samples treated with 60 μM NH<sub>4</sub>+ than for the control (121% saturation). Ammonium uptake was highest at pH 7.5 and 60 μM NH<sub>4</sub>+, with a rate of 0.526±0.002 μmol g-1 FW h-1, followed in order by the treatments of pH 8.2/60 μM NH<sub>4</sub>+, pH 7.5/4 μM NH<sub>4</sub>+, and the control (pH 8.2/4 μM NH<sub>4</sub>+). We speculated that the rates of photosynthesis and NH<sub>4</sub>+ uptake could be enhanced at a higher ammonium concentration and lower pH because CO<sub>2</sub> concentrations were increased through greater photosynthetic activity. Therefore, these findings suggest that the physiology of G. chorda populations can be improved by the interaction of optimized CO<sub>2</sub> concentrations and an adequate supply of essential nutrients such as ammonium.

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