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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.12
- 수록면
- 575 - 579 (5page)
이용수
이 논문의 연구 히스토리 (3)
2018
Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification
Nam-Yeon Kim
,
Jonghee Oh
,
Su-Heon Lee
외 3명
The Plant Pathology Journal
2018.12
학술저널
Rapid, sensitive, and specific detection of Apple stem pitting virus in pear plants by reverse transcription-recombinase polymerase amplification assay
Nam-Yeon Kim
,
Hyo-Jeong Lee
,
Rae-Dong Jeong
한국농약과학회 학술발표대회 논문집
2018.10
학술대회자료
Rapid and specific detection of Apple stem grooving virus by reverse transcription-recombinase polymerase amplification
Nam-Yeon Kim
,
Hyo-Jeong Lee
,
Rae-Dong Jeong
한국농약과학회 학술발표대회 논문집
2018.10
학술대회자료
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초록· 키워드
오류제보하기
Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcriptionrecombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an ASGV-infected plant. The major advantage of this assay is that the reaction for the target virus is completed in 1 min, and amplification only requires an incubation temperature of 42°C. This assay is a promising alternative method for pear breeding programs or virus-free certification laboratories.
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