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논문 기본 정보

자료유형
학술저널
저자정보
Jo Kyeong Ah (Department of Food Science and Technology Seoul National University of Science and Technology Seoul 01811 Republic of Korea) Kim Kyeong Jin (Department of Nano Bio Engineering Seoul National University of Science and Technology Seoul 01811 Republic of Korea) Park Soo-yeon (Department of Food Science and Technology Seoul National University of Science and Technology Seoul 01811 Republic of Korea) Jeon Jin-Young (BIO R&D Center Daesang Corp. Seoul 07789 Republic of Korea) Hwang Ji Eun (BIO R&D Center Daesang Corp. Seoul 07789 Republic of Korea) Kim Ji Yeon (Department of Food Science and Technology Seoul National University of Science and Technology Seoul 01811 Republic of KoreaDepartment of Nano Bio Engineering Seoul National University of Science and T)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제33권 제4호
발행연도
2023.4
수록면
493 - 499 (7page)
DOI
10.4014/jmb.2212.12041

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In this study we evaluated the immune-enhancing effects of β-glucan, the main component of Euglena gracilis (Euglena), and Euglena on inflammatory factor expression in RAW264.7 macrophages and ICR mice with cyclophosphamide-induced immunosuppression. Macrophages were treated with βglucan or Euglena for 48 h. The β-glucan and Euglena groups exhibited higher levels of inducible nitric oxide synthase, nitric oxide, and tumor necrosis factor (TNF)-α than the control (vehicle alone) group. Animals were fed saline and β-glucan (400 mg/kg body weight (B.W.)) or Euglena (400 or 800 mg/kg B.W.) for 19 days, and on days 17–19, cyclophosphamide (CCP, 80 mg/kg B.W.) was administered to induce immunosuppression in the ICR mouse model. CCP reduced the body weight, spleen index, and cytokine expression of the mice. To measure cytokine and receptor expression, splenocytes were treated with concanavalin A (ConA) or lipopolysaccharide (LPS) as a mitogen for 24 h. In vivo, ConA stimulation significantly upregulated the expression of interferon (IFN)-γ, interleukin (IL)-10, IL-12 receptor β1, IL-1β, and IL-2 in splenocytes from the β-glucan- or Euglena-treated groups compared with those in the splenocytes from the CCP-treated group; LPS stimulation increased the levels of the cytokines TNF-α, IL-1β, and IL-6 in splenocytes from the β-glucan- or Euglena- treated groups compared with those from the CCP-treated group, but most of these differences were not significant. These results demonstrate the effect of Euglena in ameliorating macrophages and immunosuppression in CCP-treated mice. Thus, Euglena has the potential to enhance macrophageand splenocyte- mediated immune-stimulating responses.

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