지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2023.2
- 수록면
- 21 - 39 (19page)
이용수
초록· 키워드
오류제보하기
Objectives : The purpose of this study was to investigate the anti-inflammatory effect of Goihwa-san water extract(GHS) in vitro & in vivo.
Methods : In vitro, we evaluated the anti-inflammatory effect of GHS by comparing the Raw 264.7 cells with 10, 30, 100, 300㎍/㎖ of GHS for 1 hour before Lipopolysaccharide(LPS) to the single LPS treated group. We examined the relative cell viability by MTT assay and the relative level of LPS, Loxoribine(LOX), Peptidoglycan(PGN), Flagellin(FLA)-induced NO production by using Griess reagent and measured relative iNOS protein level and COX-2 protein level by using western blot and Image analyzing system. We measured the production of TNF-α, IL-1β, and IL-6 by each ELISA kits and then measured the relative levels of IκBα, p-IκBα in whole-cell lysate fraction and NF-κB in nuclear fraction by using western blot and Image analyzing system. In vivo, we induced the paw edema by subcutaneous injection of 100㎕/rat CA and measured the swelling volume of paw by using a plethysmometer and then measured the relative iNOS protein level by using western blot.
Results : As a result, in vitro, LPS, PGN-induced NO production was significantly inhibited by pretreatment with GHS. GHS reduced LPS, PGN-induced iNOS expression, PGN-induced COX-2 expression and LPS-induced production of cytokine(TNF-α, IL-1β, IL-6). Expression of IκBα was increased by pretreatment with GHS 100㎍/㎖.
And the expression of p-IκBα and NF-κB were decreased by pretreatment with GHS 100㎍/㎖. In vivo, CA-induced inflammation rat model was used for the evaluation of the anti-inflammatory effect of GHS. 0.3 or 1.0g/㎏ of GHS significantly reduced the increases of paw swelling and iNOS expression in paw tissues.
Conclusions : These results show that GHS can decrease inflammatory response via inhibition of the NF-κB pathway in vitro. And in vivo, the anti-inflammatory effect suggest the clinical basis of GHS for the treatment of inflammatory diseases.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Environmental toxicity evaluation of fluxametamide and classification in GHS system
한국실험동물학회 학술발표대회 논문집
2018 .07
-
죽엽 열수추출물의 염증억제 효과
대한한의학방제학회지
2016 .01
-
Beneficial Therapy with Natural Anti-Inflammatory Agents and Supplements
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2021 .12
-
NF-κB 억제를 통한 대회향(大茴香)의 항염증효능
대한한의학방제학회지
2016 .01
-
LPS로 유도한 염증반응에서 해죽순의 항염증 효과
대한본초학회지(본초분과학회지)
2016 .01
-
대두, 홍삼, 진피로 구성된 발효 추출물의 항염증 효능에 관한 연구
대한본초학회지(본초분과학회지)
2015 .01
-
NF-κB 저해를 통한 브로콜리 잎 추출물의 PGE<sub>2</sub> 저해효과
대한본초학회지(본초분과학회지)
2019 .01
-
Anti-inflammatory Effect of Flower Bud and Fruit of Sweet Persimmon, Diospyros kaki T
대한의생명과학회지
2020 .06
-
산업체 유통 GHS MSDS의 영업비밀 적용실태 및 개선방안 연구
한국산업보건학회지
2016 .01
-
Suppression of Inflammatory Responses by Black Rice Extract in RAW 264.7 Macrophage Cells via Downregulation of NF-kB and AP-1 Signaling Pathways
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
NF-κB억제를 통한 淸心凉膈散의 항염증 효과
한방안이비인후피부과학회지
2019 .01
-
A Study on GHS Classification of Potassium nitrate by acute oral Toxicity
환경독성보건학회 심포지엄 및 학술대회
2017 .05
-
Anti-inflammatory activities of sinensetin on LPS-stimulated L6 skeletal muscle cells
Journal of Biomedical and Translational Research
2019 .01
-
담쟁이덩굴 추출물의 항염증 활성 연구
대한본초학회지(본초분과학회지)
2019 .01
-
H₂O₂ 처리된 LLC-PK₁세포에서 Redox Status 및 NF-κB Signaling에 대한 夏枯草의 효과
동의생리병리학회지
2016 .08
-
Exploration of targeted Anti-inflammatory drugs using LPS- or MDP-Induced inflammation models
한국실험동물학회 학술발표대회 논문집
2025 .02
-
LPS유도 염증 동물모델에서 오적산의 항염증 효과
대한한의학회지
2021 .06
-
金銀花와 黃芩이 配伍된 處方劑의 抗炎症 效果 硏究
한방안이비인후피부과학회지
2015 .01
-
재가노인의 건강상태 인지 수준에 따른 생활만족도의 영향요인
노인간호학회지
2015 .01
-
TNF-α로 유도된 HaCaT 각질형성세포의 염증반응에서 해죽순의 항염증 효과
대한본초학회지(본초분과학회지)
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0