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A feeding trial was conducted to investigate the effects of supplementation with four phosphorus (P) additives [mono calcium phosphate (MCP), dicalcium phosphate (DCP), tricalcium phosphate (TCP) and magnesium hydrogen phos phate (MHP)] on the growth, feed utilization and whole body mineral composition of juvenile olive flounders Parali chthys olivaceus. A basal diet without P supplementation was prepared as a negative control and four supplemental P sources were added at a level of 2%. Triplicate groups of fish (initial mean body weight 11 g) were fed one of the experimental diets to apparent satiation twice a day, at 08:30 and 18:00 for 10 weeks. The final body weights of fish fed the experimental diets ranged from 29.4 g (DCP) to 34.2 g (MCP) and did not differ significantly (P>0.05) among treatments. Similar tendencies were found for all parameters including weight gain (%), specific growth rate (SGR), feed conversion ratio (FCR), protein efficiency ratio (PER), feed intake (FI) and survival rate (SR). The hematocrit (%), hemoglobin (g/dL), serum inorganic P and whole body mineral composition did not differ significantly different (P>0.05) among the treatments. Therefore, dietary P addition is not necessary for juvenile olive flounder fed a fish meal-based diet.
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