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논문 기본 정보

자료유형
학술저널
저자정보
Lee Seungjin (Department of Biological Engineering, College of Engineering , Konkuk University) Reo Seung Hyeon (Department of Biological Engineering, College of Engineering , Konkuk University) Kim Seokjoon (Department of Biological Engineering, College of Engineering , Konkuk University) Kim Seokhwan (Department of Biological Engineering, College of Engineering , Konkuk University) 이은성 (건국대학교) Cha Byung Seok (Department of Biological Engineering, College of Engineering , Konkuk University) Shin Jiye (Department of Biological Engineering, College of Engineering , Konkuk University) 한진주 (건국대학교) Ahn So Min (Department of Food Science and Biotechnology , Dongguk University-Seoul) 신한승 (동국대학교) Park Ki Soo (Department of Biological Engineering, College of Engineering , Konkuk University)
저널정보
한국바이오칩학회 BioChip Journal BioChip Journal Vol.18 No.1
발행연도
2024.3
수록면
85 - 92 (8page)
DOI
10.1007/s13206-023-00130-2

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In this study, Staphylococcus aureus (S. aureus ) was detected using a system that combined direct loop-mediated isothermal amplification (LAMP) and lateral flow assays (LFA). This technology relies on sequence-specific hybridization in LFA; furthermore, it has high specificity and addresses the limitations associated with nonspecific amplification in general colori- metric LAMP. In addition, a direct boiling method was adopted to streamline DNA extraction and enable simple detection. The established technology was used to successfully detect S. aureus at a concentration as low as 10 2 colony-forming unit/ mL, without cross-reactivity with other strains. The practical applicability of this technology was demonstrated by analyzing real samples such as beef jerky, cabbage, and eggshell, which were artificially spiked with S. aureus . This developed system may be beneficial with regard to operational simplicity, short analysis time, and high detection performance, which would enable its application in point-of-care settings and as a novel platform for detecting various pathogens.

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