지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.7
- 수록면
- 395 - 404 (10page)
이용수
초록· 키워드
오류제보하기
Background: Ginsenoside Rg₁ (Rg₁) is one of the main active components in Chinese medicines, Panax ginseng and Panax notoginseng. Research has shown that Rg₁ has a protective effect on the cardiovascular system, including anti-myocardial ischemia-reperfusion injury, anti-apoptosis, and promotion of myocardial angiogenesis, suggesting it a potential cardiovascular agent. However, the protective mechanism involved is still not fully understood.
Methods: Based on network pharmacology, ligand-based protein docking, proteomics, Western blot, protein recombination and spectroscopic analysis (UV–Vis and fluorescence spectra) techniques, potential targets and pathways for Rg₁ against myocardial ischemia (MI) were screened and explored.
Results: An important target set containing 19 proteins was constructed. Two target proteins with more favorable binding activity for Rg₁ against MI were further identified by molecular docking, including mitogen-activated protein kinase 1 (MAPK1) and adenosine kinase (ADK). Meanwhile, Rg₁ intervention on H9c2 cells injured by H₂O₂ showed an inhibitory oxidative phosphorylation (OXPHOS) pathway. The inhibition of Rg₁ on MAPK1 and OXPHOS pathway was confirmed by Western blot assay. By protein recombination and spectroscopic analysis, the binding reaction between ADK and Rg₁ was also evaluated.
Conclusion: Rg₁ can effectively alleviate cardiomyocytes oxidative stress injury via targeting MAPK1 and ADK, and inhibiting oxidative phosphorylation (OXPHOS) pathway. The present study provides scientific basis for the clinical application of the natural active ingredient, Rg₁, and also gives rise to a methodological reference to the searching of action targets and pathways of other natural active ingredients.
Methods: Based on network pharmacology, ligand-based protein docking, proteomics, Western blot, protein recombination and spectroscopic analysis (UV–Vis and fluorescence spectra) techniques, potential targets and pathways for Rg₁ against myocardial ischemia (MI) were screened and explored.
Results: An important target set containing 19 proteins was constructed. Two target proteins with more favorable binding activity for Rg₁ against MI were further identified by molecular docking, including mitogen-activated protein kinase 1 (MAPK1) and adenosine kinase (ADK). Meanwhile, Rg₁ intervention on H9c2 cells injured by H₂O₂ showed an inhibitory oxidative phosphorylation (OXPHOS) pathway. The inhibition of Rg₁ on MAPK1 and OXPHOS pathway was confirmed by Western blot assay. By protein recombination and spectroscopic analysis, the binding reaction between ADK and Rg₁ was also evaluated.
Conclusion: Rg₁ can effectively alleviate cardiomyocytes oxidative stress injury via targeting MAPK1 and ADK, and inhibiting oxidative phosphorylation (OXPHOS) pathway. The present study provides scientific basis for the clinical application of the natural active ingredient, Rg₁, and also gives rise to a methodological reference to the searching of action targets and pathways of other natural active ingredients.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
4. Conclusion
References
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Ginsenoside Rg3 suppresses mast cellemediated allergic inflammation via mitogen-activated protein kinase signaling pathway
Journal of Ginseng Research
2019 .04
-
Ginsenoside Rg1 enhances the healing of injured tendon in achilles tendinitis through the activation of IGF1R signaling mediated by oestrogen receptor
Journal of Ginseng Research
2022 .07
-
Global analysis of ginsenoside Rg1 protective effects in b-amyloid-treated neuronal cells
Journal of Ginseng Research
2017 .10
-
Ginsenoside Rg1 augments oxidative metabolism and anabolic response of skeletal muscle in mice
Journal of Ginseng Research
2019 .06
-
Ginsenoside Rg1 suppresses early stage of adipocyte development via activation of C/EBP homologous protein-10 in 3T3-L1 and attenuates fat accumulation in high fat diet-induced obese zebrafish
Journal of Ginseng Research
2017 .01
-
Ginsenoside Rg1 reduces cardiac inflammation against myocardial ischemia/reperfusion injury by inhibiting macrophage polarization
Journal of Ginseng Research
2024 .11
-
A UPLC/MS-based metabolomics investigation of the protective effect of ginsenosides Rg1 and Rg2 in mice with Alzheimer’s disease
Journal of Ginseng Research
2016 .01
-
Ginsenoside Rg3 ameliorates myocardial glucose metabolism and insulin resistance via activating the AMPK signaling pathway
Journal of Ginseng Research
2022 .03
-
Longevity, tumor, and physical vitality in rats consuming ginsenoside Rg1
Journal of Ginseng Research
2023 .03
-
Ginsenoside Rg1 attenuates mechanical stress-induced cardiac injury via calcium sensing receptor-related pathway
Journal of Ginseng Research
2021 .11
-
Ginsenoside Rg1 promotes browning by inducing UCP1 expression and mitochondrial activity in 3T3-L1 and subcutaneous white adipocytes
Journal of Ginseng Research
2019 .10
-
Ginsenoside Rg5 promotes muscle regeneration via p38MAPK and Akt/mTOR signaling
Journal of Ginseng Research
2023 .11
-
Ginsenoside Rg1 Epigenetically Modulates Smad7 Expression in Liver Fibrosis via MicroRNA-152
Journal of Ginseng Research
2023 .07
-
Ginsenoside Rg1 from Panax ginseng enhances myoblast differentiation and myotube growth
Journal of Ginseng Research
2017 .10
-
Molecular signaling of ginsenosides Rb1, Rg1, and Rg3 and their mode of actions
Journal of Ginseng Research
2018 .04
-
Ginsenoside Rg1 activates ligand-independent estrogenic effects via rapid estrogen receptor signaling pathway
Journal of Ginseng Research
2019 .10
-
Rg3-enriched red ginseng extract promotes lung cancer cell apoptosis and mitophagy by ROS production
Journal of Ginseng Research
2022 .01
-
Ginsenoside Rg3 attenuates skin disorders via down-regulation of MDM2/HIF1a signaling pathway
Journal of Ginseng Research
2021 .09
-
초음파 처리에 의한 인삼꽃대 엑스의 진세노사이드 성분 변화
생약학회지(Korean Journal of Pharmacognosy)
2016 .03
-
Ginsenoside Rg3 ameliorates allergic airway inflammation and oxidative stress in mice
Journal of Ginseng Research
2021 .11
최근 본 자료
댓글(0)
0