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논문 기본 정보

자료유형
학술저널
저자정보
Khamsuk Khammool (Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand) Dell Bernard (Centre for Crop and Food Innovation, Murdoch University, 90 South St., Murdoch WA, 6150 Australia) Pathom-aree Wasu (Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand) Pathaichindachote Wanwarang (Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, ThailandCenter of Excellence in Research for Agricultur) Suphrom Nungruthai (Center of Excellence in Biomaterials, Naresuan University, Phitsanulok 65000, ThailandDepartment of Chemistry, Faculty of Science and Center of Excellence for Innovation in Chemistry, Naresu) Nakaew Nareeluk (Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, ThailandCentre of Excellence in Fungal Research, Naresuan University, Phitsan) Jumpathong Juangjun (Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, ThailandCentre of Excellence in Fungal Research, Naresu)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology Vol.34 No.5
발행연도
2024.5
수록면
1,029 - 1,039 (11page)
DOI
10.4014/jmb.2402.02008

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초록· 키워드

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This study explores beneficial bacteria isolated from the roots and rhizosphere soil of Khao Rai Leum Pua Phetchabun rice plants. A total of 315 bacterial isolates (KK001 to KK315) were obtained. Plant growth-promoting traits (phosphate solubilization and indole-3-acetic acid (IAA) production), and antimicrobial activity against three rice pathogens (Curvularia lunata NUF001, Bipolaris oryzae 2464, and Xanthomonas oryzae pv. oryzae) were assessed. KK074 was the most prolific in IAA production, generating 362.6 ± 28.0 μg/ml, and KK007 excelled in tricalcium phosphate solubilization, achieving 714.2 ± 12.1 μg/ml. In antimicrobial assays using the dual culture method, KK024 and KK281 exhibited strong inhibitory activity against C. lunata, and KK269 was particularly effective against B. oryzae. In the evaluation of antimicrobial metabolite production, KK281 and KK288 exhibited strong antifungal activities in cell-free supernatants. Given the superior performance of KK281, taxonomically identified as Bacillus sp. KK281, it was investigated further. Lipopeptide extracts from KK281 had significant antimicrobial activity against C. lunata and a minimum inhibitory concentration (MIC) of 3.1 mg/ml against X. oryzae pv. oryzae. LC-ESI-MS/MS analysis revealed the presence of surfactin in the lipopeptide extract. The crude extract was non-cytotoxic to the L-929 cell line at tested concentrations. In conclusion, the in vitro plant growth-promoting and disease-controlling attributes of Bacillus sp. KK281 make it a strong candidate for field evaluation to boost plant growth and manage disease in upland rice.

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