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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.6
- 수록면
- 310 - 317 (8page)
- DOI
- 10.1007/s13206-024-00149-z
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초록· 키워드
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This study presents a novel method for detecting plant viruses by combining a personal glucose meter (PGM)-based cascade enzymatic reaction (CER) with loop-mediated isothermal amplifi cation (LAMP). This technique exploits the consumption of deoxynucleotides (dNTPs) during the LAMP process as a substrate for CER, leading to a measurable change in glucose concentration. This change can be detected using PGM, enabling the identifi cation of the presence or absence of the target virus. This method provide a more effi cient alternative to traditional methods like ELISA and PCR. It overcomes their limi- tation in terms of laboratory equipment requirement, sensitivity, and on-site applicability. In addition, we also developed a portable diagnostic device that integrates a heating block with a glucose measurement module. By utilizing this device, the rapid and precise detection of various plant viruses, including horseradish latent virus (HRLV), onion yellow dwarf virus (OYDV), soybean yellow common mosaic virus (SYCMV), cnidium vein yellowing virus 1 (CnVYV-1), and perilla mosaic virus (PerMV), successfully achieved within 40 min. This advancement off ers a practical and cost-eff ective solution for managing plant pathogen threats in agriculture.
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