Purpose: This study aimed to understand the effects of end-of-life nursing stress and death anxiety on burnout among nurses in tertiary hospitals. Methods: Data were collected from 140 nurses who had more than one year of work experience and provided end-of-life care at a tertiary hospital in B city. Self-reported questionnaires were analyzed using descriptive statistics, independent t-tests, one-way ANOVA, Scheff? tests, Pearson's correlation coefficients, and multiple regression analyses. Results: The mean score was 3.60±0.59 (out of 5) for end-of-life nursing stress, 3.11±0.59 (out of 5) for death anxiety, and 3.13±0.61 (out of 5) for burnout. Death anxiety (β=.30, p<.001) and marital status (β=-.18, p=.033) were found to influence participants' burnout. The explanatory power of these variables for burnout was 19.2% (F=7.61, p<.001). Conclusion: Death anxiety, identified as a significant determinant of burnout among nurses in tertiary hospitals, highlights the need to develop and implement programs that prevent and alleviate death anxiety. This could, in turn, play a crucial role in reducing burnout among nurses.