지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.12
- 수록면
- 379 - 390 (12page)
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초록· 키워드
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Vibrio vulnificus is an opportunistic human pathogen with a mortality rate exceeding 50%. Human infection can occur through the consumption of raw seafood or the exposure of open wounds to contaminated water. This study aims to characterize the epidemic and virulence potentials of V. vulnificus isolates from clinical and environmental samples. We collected 35 clinical isolates between 2014 and 2022 and 42 environmental isolates between 2020 and 2022 from Jeollanam-do, South Korea. Genotyping was conducted using PCR targeting the 16S rRNA gene (rrn) and virulence-correlated gene (vcg). Multi-locus sequence typing (MLST) was performed by amplifying internal fragments of 10 house-keeping genes and concatenated sequences of 10 genes were used for phylogenetic analysis. The majority of both clinical (80%) and environmental (81%) isolates were of the C-genotype (rrnB/vcgC). MLST analysis revealed high clonal diversity, with 59 distinct sequence types (STs) identified among 77 isolates. The most prevalent ST, ST629 (n=10), was found exclusively in environmental isolates collected at low temperature (3.7~16.6℃). Phylogenetic analysis identified two arbitrarily lineages, with most isolates (70 of 77) belonging to lineage C. Clinical and environmental isolates from the warm season were dispersed across both lineages, whereas all isolates from the cold season belonged to lineage C. Given the high genetic diversity across isolate sources and genotypes, MLST-based phylogenetic analysis is challenging for predicting the pathogenic potential of V. vulnificus. This highlights the need for new genetic markers with epidemiological relevance to identify high-risk strains for public health and environmental risk management
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
CONCLUSION
REFERENCES
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