지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2025.1
- 수록면
- 22 - 33 (12page)
이용수
초록· 키워드
오류제보하기
Background: Ginseng is processed into several types such as white ginseng, red ginseng, and black ginseng, according to the processing methods such as drying, steaming, and heating. These processing conditions can change the portion of the useful ingredients. Recently, new processing method was established to develop ‘lymphanax’, an aged fresh white ginseng prepared under anaerobic condition. This aging process was revealed to increase the content of gypenoside 17 (Gyp17) as well as ginsenoside Re, known to have anti-inflammatory effects. As the next step, therefore, we aimed to investigate the anti-inflammatory activity of lymphanax using its ethanol extract of lymphanax (Lymphanax-EE).
Methods: LC-MS/MS identified the ginsenoside content of lymphanax-EE. A nitric oxide (NO) assay revealed the anti-inflammatory activity of lymphanax-EE. Pro-inflammatory gene expression was analyzed by quantitative PCR. Finally, we identified the underlying mechanism for the anti-inflammatory activity of lymphanax-EE through luciferase analysis, Western blotting, and CETSA.
Results: The LC-MS/MS analysis revealed lymphanax-EE to contain more protopanaxatriol-type ginsenosides, and Gyp17 than fresh ginseng. Lymphanax-EE (0–200 μg/ml) suppressed NO release and mRNA levels of proinflammatory cytokines such as iNOS and COX-2 in LPS-treated RAW264.7 cells. Moreover, lymphanax-EE (200 μg/ml) reduced the activity of NF-κB and phosphorylation of NF-κB signal proteins such as p65, p50, IκBα, and IKKα/β. Finally, lymphanax-EE (200 μg/ml) decreased the phosphorylation of IKKα/β induced by AKT overexpression. Among the components of lymphanax-EE, ginsenoside Re and Gyp17 were found to suppress AKT1 activity.
Conclusions: Lymphanax-EE-containing ginsenosides and Gyp17 with anti-inflammatory properties suppressed LPS-induced inflammation by reducing the NF-κB signal.
Methods: LC-MS/MS identified the ginsenoside content of lymphanax-EE. A nitric oxide (NO) assay revealed the anti-inflammatory activity of lymphanax-EE. Pro-inflammatory gene expression was analyzed by quantitative PCR. Finally, we identified the underlying mechanism for the anti-inflammatory activity of lymphanax-EE through luciferase analysis, Western blotting, and CETSA.
Results: The LC-MS/MS analysis revealed lymphanax-EE to contain more protopanaxatriol-type ginsenosides, and Gyp17 than fresh ginseng. Lymphanax-EE (0–200 μg/ml) suppressed NO release and mRNA levels of proinflammatory cytokines such as iNOS and COX-2 in LPS-treated RAW264.7 cells. Moreover, lymphanax-EE (200 μg/ml) reduced the activity of NF-κB and phosphorylation of NF-κB signal proteins such as p65, p50, IκBα, and IKKα/β. Finally, lymphanax-EE (200 μg/ml) decreased the phosphorylation of IKKα/β induced by AKT overexpression. Among the components of lymphanax-EE, ginsenoside Re and Gyp17 were found to suppress AKT1 activity.
Conclusions: Lymphanax-EE-containing ginsenosides and Gyp17 with anti-inflammatory properties suppressed LPS-induced inflammation by reducing the NF-κB signal.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
AKT-targeted anti-inflammatory activity of Panax ginseng calyx ethanolic extract
Journal of Ginseng Research
2018 .10
-
Role of ginsenosides, the main active components of Panax ginseng, in inflammatory responses and diseases
Journal of Ginseng Research
2017 .10
-
Can Endoscopic Tympanoplasty Be a Good Alternative to Microscopic Tympanoplasty? A Systematic Review and Meta-Analysis
Clinical and Experimental Otorhinolaryngology
2019 .01
-
Pharmacological and medical applications of Panax ginseng and ginsenosides : a review for use in cardiovascular diseases
Journal of Ginseng Research
2018 .06
-
Weight Loss as a Nonpharmacologic Strategy for Erosive Esophagitis: A 5-Year Follow-up Study
Gut and Liver
2018 .01
-
대두, 홍삼, 진피로 구성된 발효 추출물의 항염증 효능에 관한 연구
대한본초학회지(본초분과학회지)
2015 .01
-
Remarkable impact of steam temperature on ginsenosides transformation from fresh ginseng to red ginseng
Journal of Ginseng Research
2018 .06
-
Ginsenosides from Korean Red Ginseng ameliorate lung inflammatory responses : inhibition of the MAPKs/NF-kB/c-Fos pathways
Journal of Ginseng Research
2018 .10
-
인삼의 열처리 과정 중 생성되는 3종의 수산화진세노사이드에 대한 연구
생약학회지(Korean Journal of Pharmacognosy)
2020 .12
-
Anti-inflammatory effect of the extract of black ginseng in LPS-stimulated Raw 264.7 macrophages
한국실험동물학회 학술발표대회 논문집
2016 .08
-
Stem-and-leaf of new hydroponically-cultured ginseng cultivar K-1: A sustainable and innovative resource of ginsenosides for anti-inflammatory agents
Journal of Ginseng Research
2024 .11
-
Regulatory roles of ginseng on inflammatory caspases, executioners of inflammasome activation
Journal of Ginseng Research
2020 .05
-
JS-III-49, a hydroquinone derivative, exerts anti-inflammatory activity by targeting Akt and p38
The Korean Journal of Physiology & Pharmacology
2017 .05
-
Roles of ginsenosides in sepsis
Journal of Ginseng Research
2023 .01
-
A comparative study on chemical composition of total saponins extracted from fermented and white ginseng under the effect of macrophage phagocytotic function
Journal of Ginseng Research
2017 .07
-
Effect of hydrothermal processing on ginseng extract
Journal of Ginseng Research
2017 .10
-
Korean Red Ginseng saponin fraction exerts anti-inflammatory effects by targeting the NF-kB and AP-1 pathways
Journal of Ginseng Research
2022 .05
-
Possibility as role of ginseng and ginsenosides on inhibiting the heart disease of COVID-19: A systematic review
Journal of Ginseng Research
2022 .05
-
내시경 귀 수술의 안전성 및 유효성: 체계적 문헌고찰
대한이비인후-두경부외과학회지
2019 .01
-
Effects of Panax ginseng and ginsenosides on oxidative stress and cardiovascular diseases: pharmacological and therapeutic roles
Journal of Ginseng Research
2022 .01
이 논문의 저자 정보
이 논문과 함께 이용한 논문
UPLC-MS² combined molecular networking based discovery of nortriterpenoids from biotransformation of ginsenosides in Sanqi rhizosphere soil
Jia-Huan Shang
,
Xin-Xin Li
,
Xin-Xin Wang
외 4명
Journal of Ginseng Research
2024
.11
쉽게 간과할 수 있는 요로기계 파열에 관하여
최갑철
,
감방창
,
지해종
WITH CAP(COMPANION ANIMAL PRACTICE)
2014
.10
항산화효소의 암 예방 효과 및 발암 억제 기전
정화진
,
조윤주
,
원장원
외 1명
한국환경성돌연변이·발암원학회지
2006
.06
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-151-25-02-092298111