Purpose: Recently, as the use of medical devices has increased in most departments of general hospitals, alert fatigue among nurses has increased. This study attempted to identify factors affecting alert fatigue among nurses. Methods: Differences in general characteristics, alert fatigue, clinical nurses, burnout, self-efficacy, job satisfaction, alarm awareness, and alarm priority were analyzed using t-test and ANOVA, and Scheffé test was used for post-mortem analysis. Their correlation was analyzed using Pearson's correlation coefficient. Hierarchical multiple regression analysis was conducted to identify the factors affecting alert fatigue, clinical nurses. Results: In this study, the medical devices with the highest alert fatigue, health personnel were physiological monitors and infusion/syringe pumps. The factors affecting nurses' alert fatigue were identified as the level of alarm awareness, burn out, the effect of unnecessary alarms on work efficiency, and the nurse’s department (special departments: intensive care units, emergency room, operating room), and the explanatory power was confirmed to be 23%. Conclusion: This study identified the types of medical devices that cause alert fatigue in nurses working in special departments, general wards, and other wards. We suggested developing a medical device alarm menagement program to reduce nurses' alert fatigue.