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논문 기본 정보

자료유형
학술저널
저자정보
Jinsu Lim (Chungnam National University) Su Young Shin (Nakdonggang National Institute of Biological Resources (NNIBR)) Kihyun Kim (Chungnam National University) Hyeon Ji Yeo (Chungnam National University) Md Romij Uddin (Bangladesh Agricultural University) Tae Jin Kim (Nakdonggang National Institute of Biological Resources (NNIBR)) Min Jeong Seo (Nakdonggang National Institute of Biological Resources (NNIBR)) Sang Un Park (Chungnam National University)
저널정보
한국약용작물학회 한국약용작물학회지 한국약용작물학회지 제33권 제2호
발행연도
2025.4
수록면
117 - 124 (8page)
DOI
10.7783/KJMCS.2025.33.2.117

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Background: Tissue culture is an important strategy for metabolite accumulation in plants. More over, adventitious roots can produce high levels of phytochemicals. Actinostemma lobatum is con sumed as a medicinal plant in South Korea, China, India, and Thailand. In this study, we aimed to identify the effects of darkness and light on metabolic changes and antioxidant activity in the adventitious roots of A. lobatum.
Methods and Results: To confirm metabolic changes and antioxidant activity, adventitious roots of A. lobatum were grown under appropriate conditions and separated into dark and light treatment groups. Light conditions were found to more significantly influence the accumulation of natural pigments, such as chlorophyll a [0.136 ± 0.001 ㎎·g<sup>-1</sup> dry weight (DW)] and total carotenoids (0.047 ± 0.001 ㎎·g<sup>-1</sup> DW), than dark conditions (0.072 ± 0.001 ㎎·g<sup>-1</sup> DW and 0.026 ± 0.003 ㎎·g<sup>-1</sup> DW, respectively). Moreover, the light treatment group had substantially high contents of total phenolic, flavonoid, and rutin compounds (11.273 ± 0.291 gallic acid equivalent (GAE)·㎎·g<sup>-1</sup> DW, 9.943 ± 0.28 quercetin equivalent (QE)·㎎·g<sup>-1</sup> DW, and 2.136 ± 0.491 ㎎·g<sup>-1</sup> DW, respectively). These results revealed that light stimulates the accumulation of secondary metabolites, such as phenolics and flavonoids, and enhances antioxidant activity in the adventitious roots of A. lobatum.
Conclusions: Light conditions can considerably influence the production of health-beneficial metabolites in the adventitious roots of A. lobatum. This provides an understanding of metabolism in adventitious roots for further experimental studies on environmentally sustainable plant secondary metabolite production. In this study, we show that suitable root cultures have the potential to be used as supplements in the pharmaceutical and nutraceutical industries.

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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

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