지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 발행연도
- 2013
- 저작권
- 대전대학교 논문은 저작권에 의해 보호받습니다.
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Abstract
Objective: This study was aimed to evaluate the effects of microparticles of Socheongryong-tang (SCRT) on COPD mouse model.
Materials and Methods: The inhalable microparticles containing SCRT were produced by spray-drying with leucine as excipient, and evaluated the aerodynamic properties of the powder by Anderson Cascade Impactor (ACI) and evaluated its equivalence with SCRT extract using lipopolysaccharide (LPS) and cigarette smoking (CS) induced murine COPD model.
Results: SCRT microparticle provided desirable aerodynamic properties (fine particle fraction of 49.6±5.5% and mass median aerodynamic diameter of 4.8±0.3 ㎛). SCRT microparticle did not show mortality or clinical sign for 14 days. Also there were no significant differences in body weight, organ weights and serum chemical parameters between SCRT microparticle and intact groups. After 14 days the platelet count significantly increased compared with intact group, but its values were within normal range. Inhalation of SCRT microparticles decreased the rate of neutrophils in blood, granulocytes in peripheral blood mononuclear cell (PBMC) and bronchoalveolar lavage fluid (BALF) and level of TNF-α and IL-6 in BALF on COPD mouse model treated with LPS plus CS. This effect was verified by histological findings including immunofluorescence staining of elastin, collagen, and caspase 3 protein in lung tissue.
Conclusion: These data demonstrate that SCRT microparticles are equivalent to SCRT extract in pharmaceutical properties for COPD. This study suggests that SCRT microparticles would be potential agent of inhalation therapy for the treatment of COPD.
Objective: This study was aimed to evaluate the effects of microparticles of Socheongryong-tang (SCRT) on COPD mouse model.
Materials and Methods: The inhalable microparticles containing SCRT were produced by spray-drying with leucine as excipient, and evaluated the aerodynamic properties of the powder by Anderson Cascade Impactor (ACI) and evaluated its equivalence with SCRT extract using lipopolysaccharide (LPS) and cigarette smoking (CS) induced murine COPD model.
Results: SCRT microparticle provided desirable aerodynamic properties (fine particle fraction of 49.6±5.5% and mass median aerodynamic diameter of 4.8±0.3 ㎛). SCRT microparticle did not show mortality or clinical sign for 14 days. Also there were no significant differences in body weight, organ weights and serum chemical parameters between SCRT microparticle and intact groups. After 14 days the platelet count significantly increased compared with intact group, but its values were within normal range. Inhalation of SCRT microparticles decreased the rate of neutrophils in blood, granulocytes in peripheral blood mononuclear cell (PBMC) and bronchoalveolar lavage fluid (BALF) and level of TNF-α and IL-6 in BALF on COPD mouse model treated with LPS plus CS. This effect was verified by histological findings including immunofluorescence staining of elastin, collagen, and caspase 3 protein in lung tissue.
Conclusion: These data demonstrate that SCRT microparticles are equivalent to SCRT extract in pharmaceutical properties for COPD. This study suggests that SCRT microparticles would be potential agent of inhalation therapy for the treatment of COPD.
목차
Ⅰ.서론 1Ⅱ.실험 31.1 재 료 31.1.1 동물 31.1.2 약재 31.1.3 시약 41.2 방 법 51.2.1 흡입용 microparticle의 제조 및 공기역학적 특성 파악 51.2.2 소청룡탕 microparticle의 독성평가 61.2.2.1 투여용량의 설정 및 투여방법 61.2.2.2 일반 임상증상 및 사망동물 관찰 61.2.2.3 체중 측정 61.2.2.4 실험동물의 부검 및 장기중량 측정 61.2.2.5 혈액학적 검사 71.2.2.6 혈액 생화학적 검사 71.2.3 COPD 모델 효능 평가 71.2.3.1 폐손상 모델 71.2.3.2 혈액학적 검사 81.2.3.3 기관지폐포 세척액 분리 81.2.3.4 유세포분석 91.2.3.5 Enzyme-Linked Immunosorbent Assay (ELISA) 91.2.3.6 Hemotoxylin & Eosin 염색 101.2.3.7 면역형광염색 101.2.3.8 조직 sample의 현미경 분석 121.2.4 통계분석 12Ⅲ.결과 131.1 소청룡탕 microparticle의 공기역학적 특성 131.2 소청룡탕 microparticle의 독성평가 141.2.1 일반 임상증상 및 사망동물 141.2.2 치사율 및 LD50 141.2.3 체중 변화 141.2.4 부검시 육안소견 151.2.5 장기의 중량 변화 151.2.6 혈액학적 검사 171.2.7 혈액 생화학적 검사 191.3 COPD 모델에 대한 영향 211.3.1 혈액 내 neutrophil 비율에 대한 영향 211.3.2 PBMC 내 CD11b/Gr-1 세포 비율에 미치는 영향 221.3.3 기관지폐포 세척액 내 CD11b/Gr-1 세포 비율에 미치는 영향 231.3.4 기관지폐포 세척액 내 염증 cytokine 생성에 미치는 영향 241.3.5 조직학적 변화 261.3.6 Caspase 3 생성에 미치는 영향 271.3.7 Elastin 생성에 미치는 영향 281.3.8 Collagen 생성에 미치는 영향 29Ⅳ.고찰 30Ⅴ.결론 36
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