The current study was carried out to determine the effects of Kohlrabi and Sansa on proliferation and differentiation of pig preadipocytes and 3T3-L1 cells, aiming to seek the possibility of two plant products being used as antilipogenic agent for the livestocks and humans.
For the culture of pig preadipocytes, the cells were isolated from the backfat of the new-born pigs. Piglets were killed by CO2 asphyxiation and the subcutaneous adipose tissue was isolated and digested with collagenase. Isolated preadipocytes were seeded in DMEM/F-12 media. Twenty-four hours of after seeding, the cells were washed with DMEM/F-12(desigrated day 0). To measure the cell proliferation the cells were treated with 25ng/ml and 100ng/ml ethanol extracts of Kohlabi and Sansa and Dimethyl sulphoxide(DMSO) was used as the control group.
Kohlrabi and Sansa were treated for two days(day 0 ~ 2). The cell nember was measured on day 2 with hematocytometer after trypsin digestion of cells in culture. To measure differentiation the cells were cultured in the DMEM/F-12 containing 10% FBS and insulin(600 ng/㎖), transferrin(1 ng/㎖) and hydrocortisone(500 ng/㎖)(ITC) for 6 days(day 0 ~ 6) and the cells were treated with Kohlrabi and Sansa for two days(day 0 ~ 2). The extent of cell differentiation was assessed on day 6 by measuring activity of glycerol-3-phosphate degydrogenase(GPDH).
Tewnty five ng/ml and 100ng/ml of Kohlrabi skin decreased proliferation of pig preadipocytes by 4.59% and 17.7%, respectively compared with the control and Kohlabi fruit by 11.4% and 19.2%, respectively. Twenty five ng/ml and 100ng/ml of Sansa(China) decreased proliferation of the cells by 5.56% and 20.2%, respectively, and the Sansa(Korea) by 17.5% and 32%, respectively. No major difference was found in differentiation among treatments.
To measure the effects of Kohlrabi and Sansa on proliferation and differenetiation of 3T3-L1 cells, the cells were treated with the two products for the two days in culture, like pig preadipocytes. Kohlabi(both skin and fruit) did not show any major effect on cell proliferation. However, Twenty five ng/ml and 100ng/ml of Sansa(China) decreased cell proliferation by 24.5% and 34.3%, respectively, and 25ng/ml and 100ng/ml of Sansa(Korea) by 24.1% and 26.9% respectively. There was no major difference in cell differentiation among treatments.
In summary, the results of the current study showd that Kohlabi and Sansa decreased proliferation of pig preadipocytes, but no major effects on differentiation of the cells. Only Sansa decreased proliferation of 3T3-L1 cells, but not Kohlabi. No major difference was found in differentiation of the cells among treatments.