The purpose of the current study was to determine in vitro anticancer and preventive effects of mixed starter doenjang prepared with bamboo salts on DSS-induced colitis in mice. There were two different types of grain type mejus, which were fermented with single starter culture of Aspergillus oryzae and mixed starter cultures of Aspergillus oryzae, Bacillus subtilis and Lactococcus lactis. These mejus were mixed with different kinds of salts, which were purified salt(AP-d, MP-d), sea salt(AS-d, MS-d), one-time baked bamboo salt(A1B-d, M1B-d), three-time baked bamboo salt(A3B-d, M3B-d), and nine-time baked bamboo salt(A9B-d, M9B-d) when doenjangs were prepared. For the doenjang fermentation period of 8 weeks at 37℃, the fermentation characteristics of all groups were compared. Amino type nitrogen content and enzyme activities(protease and α-amylase) in the single starter culture samples decreased, but in the mixed starter cultures significantly increased. In DPPH free and hydroxyl radical scavenging activities of single starter culture doenjang, A9B-d (60% and 58%) showed the highest scavenging activities compared to AP-d (35% and 46%), AS-d (46% and 49%), A1B-d (48% and 52%) and A3B-d (54% and 53%) (p<0.05). Furthermore, M9B-d (78% and 69%) showed the highest scavenging activities compared to MP-d(62% and 55%), MS-d(72% and 58%), M1B-d(74% and 61%), M3B-d(76% and 66%) as well as single starter culture groups. Anticancer effects of doenjang in HT-29 and HCT-116 cancer cells indicated all groups, especially mixed starter culture doenjang prepared with bamboo salts were the highest among the others (p<0.05). Apoptosis related genes of Bax and Bcl-2 and inflammation related genes of iNOS and COX-2 were regulated by the treatment of mixed starter culture doenjangs in HT-29 cancer cells. MS-d, M1B-d, M3B-d and M9B-d increased the mRNA expression level of Bax and decreased the mRNA expression level of Bcl-2, iNOS and COX-2. The experimental ulcerative colitis is chronic inflammatory illness in colon, which did not reveal the exact reason. In this study, there were seven groups, which were normal with no-treatment, control(-) received PBS and DSS, control(+) received sulfasalazine and DSS, MP-d received MP-d and DSS, MS-d received MS-d and DSS, M1B-d received M1B-d and DSS, and M9B-d received M9B-d and DSS. The colitis was induced by 3% DSS for 7 days in 5 weeks mice. All groups with doenjang treatment were recovered from a severe inflammation of colon mucosa, but control(-) was found in many severe inflammation and edema in colon mucosa. Pro-inflammatory cytokines of serum(TNF-α, IL-6, IL-12, IL-1β and IL-17) and colon tissue(TNF-α and IL-6) significantly decreased in all groups. The mRNA expression of iNOS and COX-2 in colon tissue also decreased in all groups. These results suggest that sea salt and bamboo salts especially bamboo salt could improve fermentation characteristics and functionality of doenjang and play an important role in regulating apoptosis and inflammation related genes in the cancer cells. In addition, doenjang prepared with mixed starter and bamboo salts could have a preventive effect of colon colitis in mice.