This study was conducted to investigate preventive effects of Sulfur fed duck in vivo DSS induced colitis and AOM/DSS induced colitis-associated colon cancer. Sulfur fed duck was fed diets containing of sulfur. Extracts of Sulfur fed duck, extracts of general duck and extracts of general chicken on colitis in 2.5% DSS induced mice exhibited anti-inflammatory effects. Sulfur fed duck was ameliorated reduction of body weight and shortening of colon length and inhibited the induction of colitis by regulating IL-6 and TNF-α. It was also suppressed the expressions of iNOS and COX-2 when the samples were treated. These results suggest that anti-inflammatory effects showed much better extracts of Sulfur fed duck than extracts of general duck. Extracts of Sulfur fed duck(1 mg/mL) exhibited excellent preventive effects on DSS induced colitis. Extracts of Sulfur fed duck in medicine had preventive effect on and AOM/DSS induced colitis-associated colon cancer(CAC). Extracts of Sulfur fed duck in medicine was body weight and shortening of colon length and inhibited the regulating induction of IL-6 and TNF-α. It was also suppressed the expressions of iNOS and COX-2 when the samples were treated. Extracts of Sulfur fed duck in medicine was better than extracts of general duck in medicine. It was decreased the serum concentration of IL-6 and TNF-α and suppressed the expressions of iNOS and COX-2. Extracts of Sulfur fed duck in medicine(3mg/mL) showed the best protective effects on and exhibited the highest anti-inflammatory effects on AOM/DSS induced colitis-associated colon cancer(CAC). In addition, p21, p53 expressions of extracts of Sulfur fed duck in medicine treatment significantly in creased compared to extracts of general duck in medicine(p<0.05). These results suggest that extracts of Sulfur fed duck in medicine(3 mg/mL) exhibited excellent preventive effects on in vivo AOM/DSS induced colitis-associated colon cancer(CAC). Response from more general results was extracts Sulfur fed duck and extracts Sulfur fed duck in medicine better than extracts general duck and extracts general duck in medicine. Because of Sulfur fed duck extracts and Sulfur fed duck in medicine contain more sulfur than general duck extracts and general duck in medicine. So, it was suggest repression inflammation and cancer by sulfur. This study is foundation excellent preventive effects and proof the excellence scientifical by extracts sulfur fed duck.