지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 양우철
- 발행연도
- 2013
- 저작권
- 동국대학교 논문은 저작권에 의해 보호받습니다.
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이 논문의 연구 히스토리 (3)
2013
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초록· 키워드
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For identifying the cause of neuropathies such as Alzheimer''s disease, Parkinson''s disease and stroke, many research groups need a large amount of nerve cells. However it’s difficult to get the nerve cells from human, so many research groups use the SH-SY5Y neuroblastoma cells in vitro model. Nowadays, atomic force microscopy(AFM) is utilized in many studies for measuring the structural information and physical characteristics of soft material. So in this study, SH-SY5Y cells were chosen as the experimental specimens and a detailed surface potential by Kelvin Probe Force Microscopy(KPFM) and measurement of cell membrane hardness by Force spectroscopy before and after differentiating neuronal cell was carried out. And also plan to detect D-glutamic acid which is one of neurotransmitter emitted from differentiated SH-SY5Y cell by using AFM topography image. SH-SY5Y cells were differentiated by Retinoic acid(RA) treatment and differentiated SH-SY5Y cells are determined by western blot method. Cells were fixed by using 3.7% formaldehyde aqueous solution and then dried. KPFM, Force Spectroscopy and AFM are one of SPM system application. From KPFM images, we can conclude that when SH-SY5Y cells differentiate, their surface potential values are decreased. And hardness value of SH cells membrane after differentiation decreased about 25% than that without differentiation. In measurement of detecting neurotransmitter, we can detect D-glutamic acid by using antigen-antibody binding force. Thus in this study, SPM method gives us a useful guideline for SH cell differentiation. We expect that this method can be also extended for the application of differentiation from Mesenchymal stem cell(MSC) to nerve cell.
목차
List of Figures = ⅥList of Tables = Ⅶ1장 서론 = 12장 배경이론 = 42.1 KPFM 측정원리 = 42.2 Force spectroscopy 측정원리 = 83장 실험방법 = 113.1 시료준비 = 113.1.1 세포배양 = 113.1.2 SPM 측정을 위한 시료 처리단계 = 133.2 SPM 측정 = 163.2.1 KPFM 측정과정 = 163.2.2 Force spectroscopy 측정과정 = 163.2.3 AFM 측정과정 = 194장 실험결과 및 분석 = 204.1 분화 전 후 SH-SY5Y cell의 surface potential 비교 = 204.2 분화 전 후 SH-SY5Y cell의 hardness 비교 = 294.3 분화된 SH-SY5Y cell의 신경전달물질 검출 = 335장 결론 = 396장 추가연구 = 407장 참고문헌 = 42
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