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논문 기본 정보
- 자료유형
- 학위논문
- 저자정보
- 지도교수
- 이장훈
- 발행연도
- 2014
- 저작권
- 경희대학교 논문은 저작권에 의해 보호받습니다.
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Objective : This study was designed to investigate the effect on lipoapoptosis of Alisma orientale extract against free fatty acid-induced cellular injury.
Methods : HepG2 cells were used in vitro model. HepG2 cells were treated with free fatty acids to generate a cellular model of nonalcoholic fatty liver disease(NAFLD). Using this cellular model, the anti-apoptotic effect and reducing steatosis of Alisma orientale extract against free fatty acid-induced cellular injury was evaluated by measuring steatosis and apoptosis.
Result : Alisma orientale extract significantly attenuated free fatty acid-induced intracellular steatosis. Alisma orientale extract inhibited free fatty acid-mediated activation of pJNK, PUMA, Bax, Caspase-3,-9, apoptotic kinases that are correlated with NAFLD. And Alisma orientale extract promoted Bcl-2, a anti-apoptotic protein.
conclusion : From the above, the Alisma orientale extract decreased the hepatocyte steatosis and had the hepatocelluar protective effect by the regulation of apoptosis-related protein. It proposes the possibility of Alisma orientale extract to the treatment of nonalcoholic fatty liver disease in clinics.
Methods : HepG2 cells were used in vitro model. HepG2 cells were treated with free fatty acids to generate a cellular model of nonalcoholic fatty liver disease(NAFLD). Using this cellular model, the anti-apoptotic effect and reducing steatosis of Alisma orientale extract against free fatty acid-induced cellular injury was evaluated by measuring steatosis and apoptosis.
Result : Alisma orientale extract significantly attenuated free fatty acid-induced intracellular steatosis. Alisma orientale extract inhibited free fatty acid-mediated activation of pJNK, PUMA, Bax, Caspase-3,-9, apoptotic kinases that are correlated with NAFLD. And Alisma orientale extract promoted Bcl-2, a anti-apoptotic protein.
conclusion : From the above, the Alisma orientale extract decreased the hepatocyte steatosis and had the hepatocelluar protective effect by the regulation of apoptosis-related protein. It proposes the possibility of Alisma orientale extract to the treatment of nonalcoholic fatty liver disease in clinics.
목차
Ⅰ. 緖 論 1II. 實 驗 31. 재료 31) 약재 및 추출 32. 방법 31) 세포배양 32) 간세포에 대한 약물처리 43) MTT assay 44) Oil-Red-O staining 45) Real-time PCR 46) Western blot 53. 통계분석 5Ⅲ. 結 果 61. MTT assay 61) 유리지방산이 cell viability에 미치는 영향 62) 택사 추출물이 cell viability에 미치는 영향 62. Oil-Red-O staining을 통한 세포내 지방의 양적 분석 83. 택사 추출물이 pJNK 활성에 미치는 영향 104. 택사 추출물이 PUMA 활성에 미치는 영향 125. 택사 추출물이 Bax, Bcl-2 활성에 미치는 영향 156. 택사 추출물이 caspase 활성에 미치는 영향 17Ⅳ. 考 察 19Ⅴ. 結 論 24Ⅵ. 參 考 文 獻 26ABSTRACT 32
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